Root > Products > StarGate Expression Vectors > Acceptor vectors > Mammalia > pESG-IBA and pCSG-IBA vectors for high level expression in mammalian cells

pDSG-IBA vectors

  • Optimized for MEXi, Mammalian Expression IBA, and are also suitable for other mammalian cell cultures
  • High level constitutive expression in mammalian cells by the CMV promoter
  • Extrachromosomal replication due to Epstein Barr Virus replication origin (oriP) - requires chromosomal expression of the EBNA-1 gene, e.g. like in MEXi 293E cells
  • ColEl ori and ampicillin resistance gene support propagation in E. coli
  • BM40 option for secretion of protein into the medium

pESG-IBA vectors

  • High-level constitutive expression in mammalian cells by the CMV promoter
  • Neomycin resistance for generation of stable cell lines
  • ColEl ori and ampicillin resistance gene support propagation in E. coli
  • BM40 option for secretion of protein into the medium

pCSG-IBA vectors

  • High-level constitutive expression in mammalian cells by the CMV promoter
  • extrachromosomal replication due to Epstein Barr Virus replication origin (oriP) and EBNA-1
  • prolonged expression of the inserted GOI under G418 selection without the need for making stable cell lines
  • ColEl ori and ampicillin resistance gene support propagation in E. coli
  • BM40 option for secretion of protein into the medium