Just relax - and leave your lab work to us!

IBA´s wide-range product portfolio is supplemented by a comprehensive custom service for recombinant protein production subdivided in

Cloning –  of the desired DNA  into an expression vector with selected features

Protein expressionof the desired expression construct in E. coli, HEK293 or CHO cells

Protein purification –  via affinity tags (Strep-tag®, Twin-Strep-tag® and 6xHis-tag)

Further options can be provied on request

General remarks about IBA´s custom services

Framework Agreement and Licensing

General remarks about IBA’s cloning, protein expression and purification services

  • Cloning is planned in close cooperation with the client. For example, we can introduce specific point mutations (such as additional restriction sites) into the termini of a gene via PCR cloning.
  • The original control sequencing print-outs of the gene insert can be provided on request. IBA does not guarantee that the encoded gene will be expressed.
  • For difficult gene syntheses prices and delivery times are adapted accordingly;  IBA is not obliged to accept difficult gene syntheses.
  • If for any reason a successful PCR product cannot be obtained from a template supplied by the customer and/or the sequence information, a basic fee will be charged for our cloning attempts only.
  • In case, the Strep- or 6xHistidine tag was used, the purified protein is supplied as eluted from the Strep-Tactin® or Ni-NTA affinity column in elution buffer containing desthiobiotin or imidazole, respectively.
  • Protein concentrations are determined using the theoretical extinction coefficient at 280 nm of the recombinant protein, which is determined by the addition of the extinction coefficients of the tryptophane and tyrosine residues present in the recombinant protein or by a colorimetric assay according to Bradford, with BSA as reference protein.
  • The original control sequencing print-outs of the gene insert can be provided on request. IBA does not guarantee that the encoded gene will be expressed.
Integrity, secrecy and licensing

Our custom service requires the exchange of sensitive data. Therefore, all proprietary information and intellectual property transferred to IBA/IBA Biologics is kept absolutely confidential and is covered by a secrecy agreement if requested.
As a service provider we do not intend to claim any rights on proteins which we manufacture for our clients as long as the proteins are used for in-house research purposes only. A separate license for use of the Strep-tag® technology is necessary, however, if direct commercialization of ordered proteins is intended. This is also the case if clients use the Strep-tag® technology for the production of recombinant proteins in their own facilities with subsequent commercialization. IBA is authorized and ready to grant licenses for those purposes. Information about licenses for commercial use of 6xHistidine-tag proteins is available from QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

IBA is not obliged to accept orders. Shipping costs are charged separately.

IBA patents, licensing and trademarks

Strep-Tactin® is covered by US6103493 and patent applications in Europe; the tetracycline promoter based prokaryotic expression system is covered by US5849576 and EP759997; Streptamer® technology for T-cell purification and Twin-Strep-tag® technology are protected by US 7,981,632 and further patent applications owned by IBA. Purchase of reagents related to these technologies from IBA provides a license for non-profit and in-house research use only. Cloning, expression or purification or other applications of above mentioned technologies for commercial use require a separate license from IBA. A license may be granted by IBA on a case-by-case basis, and is entirely at IBA’s discretion. Please contact IBA for further information on licenses for commercial use. Strep-tag®, Strep-Tactin®, Streptamer®, Twin-Strep-tag® and StarGate® are registered trademarks of IBA GmbH.