Protein:protein-interactions (PPI) govern almost all important processes in living organisms. Thus, their rapid and accurate determination and investigation is a major challenge in life sciences.
Based on its Twin-Strep-tag® (formerly known as One-STrEP)/Strep-Tactin® system IBA provides optimal solutions with its different determination systems for protein:protein-interaction analysis based on the procedure described in Rigaut et al., 1999.
"Protein Complex Analysis combining a one-step co-purification with a mass spectrometry approach"
Rigaut et al., 1999
This is the basic principle for affinity tag based protein complex isolation and protein interaction analysis. The further developed determination systems are described in the following sections: