Root > Products > Magnet Assisted Transfection > MATra reagents > Technical information about Magnet Assisted Transfection (MATra)

MATra Specifications

Matra is a fast, easy-to-handle, gentle technology to transfect any type of cell at different phases of cell confluence.

MATra is applicable at different phases of cell confluence, however in general we recommend 50-70% confluence (in some systems a higher visual confluence may result in higher Magnet Assisted Transfection rates).
MATra is non-toxic at recommended amounts in most cell systems. Still, if higher DNA/MATra or Enhancer amounts are used a medium change is advised within 2 hours.

Easy protocol (example with MATra-A Reagent)

  1. Dilute nucleic acid in medium
  2. Add magnetic nanoparticles (MATra-A Reagent)
  3. Incubate 20 - 30 minutes
  4. Add medium to adherent cells (2 - 4 x 105 cells)
  5. Add nucleic acid/nanoparticle solution
  6. Place culture plate onto magnet plate
  7. Incubate 15 minutes
  8. Remove magnet plate

MATra assay formats

Formats Volume of MATra-A or
MATra-si Reagent
recommended per well [µl]
Transfections per 200 µl vial
* 156 µl MATra-A are required for 500 cm2; please titrate to optimize for your application.    
96 well plate 0.1 2000
48 well plate 0.3 667
24 well plate 0.6 333
12 well plate 1.2 167
6 well plate 3 67
60 mm dish 6.6 30
100 mm dish 17.2 12
T-75 flask 23.5 9
25 x 25 cm plate 156 1*

Low vector doses

High transfection efficiencies using low vector doses are achieved using magnet assisted transfection-matra technology.
Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours.