To order please visit the New IBA Oligo Shop.

Azide availability for multiple nucleic acid labeling via Click-Chemistry is indicated by +

Fluorescent dyes Abs [nm] Em [nm] ε [cm-1M-1] Cat. no. Azide Comments
Pyrene 340 376 43,000 5-0312-6XX + Extensive fluorescence lifetime
Pyrene
  • Pyrene is excited by UV light (340 nm) and has an extensive fluorescence lifetime
  • Excited-state dimers (excimers) can form with fluorescence emission shifted to longer wavelengths than that of the monomer (this property can be utilized in nucleic acid hybridization experiments)
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-Pyrene
Abberior® FLIP 565 314 580 51,000 5-0310-0XX - Photoswitchable dye
  • Photoswitchable dye: can be reversibly switched between a fluorescent and non-fluorescent state
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Alexa Fluor® 350 345 440 19,000 5-0316-0XX - Brighter fluorescence and photostability than others
AF350

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.)
Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • structure on the right: Alexa Fluor® 350 Carboxylic Acid, Succinimidyl Ester
Eterneon™ 350/430 350 430 15,220 5-0318-0XX - A new generation of ultra-photostable labels
  • Stokes shifts up to 180 nm
  • Cover the entire spectrum
  • Extremely photostable
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by baseclick GmbH 
Eterneon™ 350/455 350 455 27,300 5-0318-0XX + A new generation of ultra-photostable labels
  • Stokes shifts up to 180 nm
  • Cover the entire spectrum
  • Extremely photostable
  • Available for standard NHS coupling as well as for the Click-Chemistry providing highly efficient and reliable oligonucleotide labeling
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by baseclick GmbH
Dy® 350 353 432 19,000 5-0311-7XX + Soluble in water, methanol, ethanol, DMF, DMSO
  • Azide available for click chemistry
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 350 XL 349 610 16,000 5-0311-7XX - UV-Megastoke
DY-350XL-Structure
  • Exceptional Stokes-Shift (UV-Megastoke)
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 360 XL 362 459 25,000 5-0311-7XX - UV-Megastoke
DY-360XL structure
  • Bright blue emission (QY 0.52 in PBS)
  • UV-Megastoke
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 370 XL 368 473 13,200 5-0311-7XX - UV-Megastoke
DY-370XL-Structure
  • Exceptional Stokes-Shift (UV-Megastoke)
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 375 XL 376 543 16,000 5-0311-7XX - UV-Megastoke
DY-375XL-Structure
  • Exceptional Stokes-Shift (UV-Megastoke)
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 380 XL 381 510 22,000 5-0311-7XX - UV-Megastoke
DY-380XL-Structure
  • Exceptional Stokes-Shift (UV-Megastoke)
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Eterneon™ 384/480 384 480 18,000 5-0318-0XX + A new generation of ultra-photostable labels
  • Stokes shifts up to 180 nm
  • Cover the entire spectrum
  • Extremely photostable
  • Available for standard NHS coupling as well as for the Click-Chemistry providing highly efficient and reliable oligonucleotide labeling
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by baseclick GmbH 
Atto® 390 390 479 24,000 5-0311-7XX + Ideally suited for bioanalytical applications
Atto390
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Azide available for Click-Chemistry
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® 390
Dy® 395 XL 396 572 20,000 5-0311-7XX - UV-Megastoke
DY-395XL-Structure
  • Exceptional Stokes-Shift (UV-Megastoke)
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Eterneon™ 393/523 393 523 27,680 5-0318-0XX + A new generation of ultra-photostable labels
  • Stokes shifts up to 180 nm
  • Cover the entire spectrum
  • Extremely photostable
  • Available for standard NHS coupling as well as for the Click-Chemistry providing highly efficient and reliable oligonucleotide labeling
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by baseclick GmbH
Eterneon™ 394/507 394 507 30,000 5-0318-0XX + A new generation of ultra-photostable labels
  • Stokes shifts up to 180 nm
  • Cover the entire spectrum
  • Extremely photostable
  • Available for standard NHS coupling as well as for the Click-Chemistry providing highly efficient and reliable oligonucleotide labeling
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by baseclick GmbH
Dy® 405 400 423 32,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 405 401 424 34,000 5-0316-0XX - Better results in Fluorescence Correlation Spectroscopy
AF405

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 405 can be 5', 3' or internally coupled to the oligo. Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Structure on the right: Alexa Fluor® 405 carboxylic acid, succinimidyl ester
Dy® 415 418 467 34,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
Dy415
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 415
Dy®Q 425 425 none 25,400 5-0311-7XX - Polar water soluble quencher
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® 425 436 484 45,000 5-0311-7XX + Low triplet formation
Atto425
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-Atto® 425
Alexa Fluor® 430 433 541 16,000 5-0316-0XX - Brighter fluorescence and photostability than others
AF430

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 430 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • has large Stokes shift
  • Structure on the right: 5'-Alexa Fluor® 430
Atto® 430 LS 433 547 32,000 5-0311-7XX + Extraordinary large Stokes shift
  • Extraordinary large Stokes shift of 114 nm
  • Anionic dye
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Product of ATTO-TEC GmbH
Dy® 430 444 487 35,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
Folie1
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 431 442 496 35,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
Folie1
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Abberior® STAR 440SXP 432 511 33,000 5-0310-0XX - Dye with large Stokes shift
  • Phosphorylated analogue of Abberior STAR 440SX with improved stability
  • Ideal for STED and well suited for confocal microscopy
  • Long Stokes' shift (~80 nm) for 2-color applications
  • High water solubility
Atto® 465 453 508 75,000 5-0311-7XX - Good water solubility
Atto465
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® 465
Abberior® STAR 470SXP 467 598 25,000 5-0310-0XX - Designed for STED microscopy
  • Phosphorylated analogue of Abberior STAR 470SX with improved stability
  • Ideal depletion behaviour in STED microscopy ~750 nm
  • 2-color labeling partner with STAR 635P for 2-color STED microscopy
  • Produced by Abberior
  • Can be 5', 3' or internally coupled to the oligo
Dy® 478 478 518 90,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dabcyl 478 none 32,000 5-0311-0XX + Universal quencher, double labelling
Dabcyl
  • Universal quencher in combination with FAM, HEX, TET, TAMRA and other dyes
  • Does not emit fluorescence and thus avoids overlapping detection signals
  • Double labeling possible with 3'-Dabcyl and any other dyer
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-Dabcyl
Eterneon™ 480/635 480 635 29,900 5-0318-0XX + A new generation of ultra-photostable labels
  • Stokes shifts up to 180 nm
  • Cover the entire spectrum
  • Extremely photostable
  • Available for standard NHS coupling as well as for the Click-Chemistry providing highly efficient and reliable oligonucleotide labeling
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by baseclick GmbH 
Dy® 485 XL 485 560 50,000 5-0311-7XX + For microarray experiments, FISH microscopy, gel electrophoresis
Dy485XL
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Has large Stokes shift
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 485 XL
Chromeo™ 488 488 517 73,000 5-0310-0XX + Narrow emission and absorption bands for use in STED microscopy
  • Soluble in water, DMSO, methanol
  • Optimal photostability
  • Bright fluorescence
  • Low background
  • pH insensitive
  • Azide available for Click-Chemistry
  • Chromeo™ is a trademark of Active Motif
Oyster® 488 494 518 80,000 5-0315-0XX + New class of cyanine dyes
Oyster
  • New class of cyanine dyes
  • High photostability
  • Bright fluorescence
  • Produced by Luminartis
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Oyster® 488
Dy® 490 491 515 73,000 5-0311-7XX + Suitable for flow-cytometry, FisH microscopy, gel electrophoresis
  • Soluble in water and methanol
  • pH-stable emission
  • Azide available for Click-Chemistry
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® 490 LS 496 661 40,000 5-0311-7XX + Extraordinary large Stokes shift
  • Extraordinary large Stokes-shift of 165 nm thus the emission spectrum is almost completely separated from the absorption spectrum
  • Anionic dye
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Product of ATTO-TEC GmbH
Fluorescein-5-EX 491 515 86,000 5-0311-2XX - More hydrophilic than FAM-5-isomer
5FAM5EX

Fluorescein-EX, 5-isomer (FAM-EX-5)

  • Extended linker arm between oligonucleotide and dye
  • More hydrophilic than FAM, 5-isomer
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'- FAM-EX-5
BHQ-0 493 none 34,000 5-0311-2XX - Optimized for FRET based quenching
5BHQ0

Black Hole QuencherTM dye

  • Robust dark quencher compatible with ammonium hydroxide deprotection
  • Excellent coupling efficiency, 
  • Non-fluorescent with large extinction coefficients
  • Also used for static quenching
  • Can be 5' or 3' coupled to the oligo
  • Structure on the right: 5'-BHQ-0 Phosphoramidite*

* Structure used with permission of Glen Research Corporation

FAM, 5-isomer 494 520 78,000 5-0311-XXX + Used in applications such as DNA sequencing, GeneScan analysis, in situ hybridization, Real-time PCR, FRET…

Fluorescein

  • High quantum yields
  • Excitation maximum in close proximity to the 488 nm spectral line of the Argon-Ion Laser
  • Used in applications such as DNA sequencing, GeneScan analysis, in situ hybridization, Real-time PCR, FRET, Molecular Beacons and many others
  • Double labelings possible with 3'-or 5'-Fluorescein and any other dye or marker
  • Can be introduced as amidite (isomeric mixture) and post synthetically as mixed, 5- or 6-isomer
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'- FAM
Dy® 495 493 521 70,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy495
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 495
Chromeo™ 494 494 628 55,000 5-0310-0XX + Large stokes shift
  • Soluble in DMF, CHCl3, methanol, ethanol
  • Photostability
  • Low background
  • pH insensitive
  • Azide available for Click-Chemistry
  • Chromeo™ is a trademark of Active Motif
Alexa Fluor® 488 496 519 71,000 5-0316-0XX + Brighter fluorescence and photostability than others
AF488

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 488 can be 5', 3' or internally coupled to the oligo
Oligonucleotides labeled with Alexa Fluor have many advantages:

  • Brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • Ppotentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • Fluorescence is stable from pH 4 to pH 10
  • Azide available for Click-Chemistry
  • Structure on the right: 5'- Alexa Fluor® 488
Atto® 495 495 527 80,000 5-0311-7XX - Ideally suited for bioanalytical applications
Atto495
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® 495
FAM, 6-isomer 496 516 83,000 5-0311-XXX + Used in applications such as DNA sequencing, GeneScan analysis, in situ hybridization, Real-time PCR, FRET…
FAM6isomer

Fluorescein

  • High quantum yields
  • Excitation maximum in close proximity to the 488 nm spectral line of the Argon-Ion Laser
  • Used in applications such as DNA sequencing, GeneScan analysis, in situ hybridization, Real-time PCR, FRET, Molecular Beacons and many others 
  • Double labelings possible with 3'-or 5'-Fluorescein and any other dye or marker
  • Can be introduced as amidite (isomeric mixture) and post synthetically as mixed, 5- or 6-isomer
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right:  FAM 6 isomer
IBApy 493/503 500 509 79,000 5-0310-1XX - Spectral properties that are insensitive to solvent polarity and pH
BODIPY

IBApy fluorophoresIBApy fluorophores span the complete visible spectrum and have spectral characteristics which are superior over commonly used dyes.

Advantages: 

  • Higher quantum yields
  • Narrower emission bandwidths
  • Greater photostability
  • Insensitive to pH changes
  • Useful for DNA sequencing because the dye exhibits minimal effect on the mobility of the fragment during electrophoresis
  • Not available in the US
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Basic structure of the IBApy fluorophore
Dy® 480 XL 500 630 50,000 5-0311-7XX + For protein labeling, microarray experiments, FISH microscopy, gel electrophoresis
Dy480xl
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy 480 XL
Atto® 488 501 523 90,000 5-0311-7XX + Low triplet formation
Atto488
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Almost identical spectrophysical properties as Alexa Fluor® 488
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto 488
Abberior® STAR 488 501 524 86,000 5-0310-0XX - Latest development for STED microscopy
  • Soluble in water, alcohols, acetonitrile, DMSO, DMF
  • Produced by Abberior
  • Can be 5', 3' or internally coupled to the oligo
IBApy FL 502 510 82,000 5-0310-1XX - Narrow emission bandwidth, resulting in a higher peak intensity than fluorescein
5BODIPYFl

The green fluorescent IBApy FL fluorophore has several characteristics that make it potentially superior to fluorescein.

These include:

  • High extinction coefficient
  • High fluorescent quantum yield (often approaching 1.0, even in water)
  • Spectral properties that are insensitive to solvent polarity and pH 
  • Narrow emission bandwidth, resulting in a higher peak intensity than fluorescein
  • Little or no spectral overlap with longer wavelength dyes such as tetramethyl-rhodamine and Texas Red, making it useful for multicolor applications
  • Greater photostability than fluorescein in some environments
  • Lack of ionic charge
  • Not available in the US
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5' IBApy FL
Abberior® CAGE 500 511 525 50,000 5-0310-0XX - Photoactivatable dye
  • Photoactivatable: dye is initially colorless and nonfluorescent
  • When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye with spectral properties essentially identical to Abberior® STAR 512
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Dy®Q 505 504 none 39,000 5-0311-7XX - Polar water soluble dark quencher
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 505 505 530 80,000 5-0311-7XX - Rhodamine 110 based
Dy505
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-Dy 505, 6 isomer
Dy® 505-X 505 530 85,000 5-0311-7XX + Similar to Rhodamine
  • MegaStokes Dyes (XL)
  • Produced by Dyomics
  • Can be 5', 3' or internally coupled to the oligo
Rhodamine 110X 505 530 85,000 5-0313-3XX - Higher photostability than Rhodol GreenTM and Oregon GreenTM 488
Rh110x
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-Rhodamine 110X
Dy® 510 XL 509 590 50,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Sy510XL
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 510 XL
Dy® 511 XL 510 595 47,000 5-0311-7XX - High photostability, UV-Megastoke
DY-511XL Structure
  • High photostability
  • Very hydrophilic
  • MegaStoke-Dye
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Atto® 514 511 533 115,000 5-0311-7XX + Highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.
Atto514
  • Strong absorption
  • High fluorescence quantum yield
  • Exceptional thermal and photostability
  • Excellent water solubility
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto 514
Eterneon™ Green 515 Azide 515 539 50,000 5-0318-0XX + Azide suitable for Click-Chemistry
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Atto® 520 516 538 110,000 5-0311-7XX + Ideally suited for bioanalytical applications
Atto520
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto 520
Alexa Fluor® 514 517 542 80,000 5-0316-0XX - Brighter fluorescence and photostability than others
AF514

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 514 can be 5', 3' or internally coupled to the oligo. Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Structure on the right: Alexa Fluor® 514
Dy® 481 XL 515 650 50,000 5-0311-7XX + Negatively charged with good water solubility
Dy481XL
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy 481 XL
JOE, 6-isomer 520 548 71,000 5-0311-7XX + High quantum yields and low pH sensitivity
  • Routinely used for oligonucleotide labeling and DNA-Sequencing
  • High quantum yields and low pH sensitivity
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'-JOE, 6-isomer
TET 519 539 98,000 5-0314-2XX + Can be combined with any available dye in varied positions
5TET
  • Used in multicolor applications such as the 377DNA sequencer in Genescan analysis
  • Only at the 5'-end
  • Can be combined with any available dye in varied positions
  • Can be 5' coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5' TET
Dy® 520 XL 520 664 50,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy520XL
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 520 XL
Abberior® STAR 520SXP 515 612 60,000 5-0310-0XX - Extremely suitable for STED applications
  • New member of an entirely new class of dyes
  • First commercially available phosphorylated fluorescent dye
  • Abberior® STAR520SXP can substitute dyes like Dy® 520 and Dy®521
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Carboxy-rhodamine 6G, 5-isomer 524 557 108,000 5-0310-5XX + High quantum yield and photostability
Carboxyrhodamine6G5isomer
  • High quantum yield and high photostability
  • The absorption maximum is close to the 514 nm spectral line of argon-ion lasers
  • Superior electrophoretic and spectroscopic properties to JOE-6 dye
  • Used in sequencing
  • Available as mixed, 5-and 6-isomer
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'-Carboxyrhodamine 6G, 5-isomer
Dy® 521 XL 523 668 50,000 5-0311-7XX - Negatively charged with good water solubility
Dy521XL
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH  
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 521 XL
Eterneon™ Yellow 530 Azide 529 561 54,000 5-0318-0XX + Azide suitable for Click-Chemistry
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Alexa Fluor® 532 532 553 81,000 5-0316-0XX - Brighter fluorescence and photostability than others
AF532

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 532 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Structure on the right: Alexa Fluor® 532
Atto® 532 532 553 115,000 5-0311-7XX + Ideally suited for bioanalytical applications
Atto532
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto 532
Abberior® CAGE 532 533 541 82,000 5-0310-0XX - Photoactivatable dye
  • Photoactivatable: dye is initially colorless and nonfluorescent
  • When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Dy® 530 539 561 100,000 5-0311-7XX + Soluble in water and methanol
  • Produced by Dyomics GmbH
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
BHQ-1 534 none 34,000 5-0317-1XX - Multiplex two, three and four dye combinations in one reaction tube without cross-talk between channels
BHQ1

Black Hole QuencherTM dye

  • Robust dark quencher compatible with ammonium hydroxide deprotection
  • Excellent coupling efficiency
  • Non-fluorescent with large extinction coefficients
  • Also used for static quenching
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-BHQ-1 Phosphoramidite*

* Structure used with permission of Glen Research Corporation

Atto® Rho6G 535 560 115,000 5-0311-7XX + Extraordinary high fluorescence quantum yield
  • New rhodamine dye
  • Strong absorption
  • High thermal and photostability
  • Very little triplet formation
  • Highly suitable for single-molecule detection applications and high-resolution microscopy
  • Moderately hydrophilic
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
HEX 537 556 99,000 5-0311-4XX + Can be combined with any available dye in varied positions
HEX
  • Used for multicolor applications, e.g. the 377 DNA sequencer in Genescan analysis
  • Can be combined with any available dye in varied positions
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'- HEX
Atto® 540Q 542 none 105,000 5-0311-7XX + Ideally suited for bioanalytical applications
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by ATTO-Tec
Atto® 542 542 562 12,000 5-0311-7XX + Hydrophil, High photostability
  • Strong absorption and high photostability
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Product of ATTO-TEC GmbH
Dy® 547P1 551 565 15,000 5-0311-7XX - Enhanced water solubility and polarity
  • Enhanced water solubility and polarity
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy®Q 1 543 none 48,000 5-0311-7XX - Soluble in water, methanol, DMF
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Eterneon™ Yellow 550 Azide 545 565 15,000 5-0318-0XX + Alternative dye for Cyanine 3
  • Alternative dye for Cyanine 3
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Abberior® CAGE 552 552 574 66,000 5-0310-0XX - Photoactivatable dye
  • Photoactivatable: Nonfluorescent and nearly colorless substance which, upon photolysis at 360−440 nm, readily transforms into the deeply red and highly fluorescent dye
  • High brightness & photostability
  • Product of Abberior GmbH
Dy® 554 551 572 100,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
TAMRA , 5-isomer 546 579 91,000 5-0314-0XX + Used as a quencher in Fluorescence Resonance Energy Transfer applications (FRET)
TAMRA5isomer

Tetramethylrhodamine

  • Widely used in non-radioactive DNA sequencing
  • High photostability
  • Used for in situ hybridization applications
  • Used as a quencher in Fluorescence Resonance Energy Transfer applications (FRET)
  • Available as 5- or 6-isomer
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'-TAMRA, 5-isomer
Dy® 555 547 572 100,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Can be 5', 3' or internally coupled to the oligo
  • Produced by Dyomics GmbH
Eterneon™ Yellow 555 Azide 551 564 77,000 5-0318-0XX + Alternative dye for Cyanine 3
  • Alternative dye for Cyanine 3
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Dy® 556 548 573 100,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Chromeo™ 546 545 561 96,800 5-0310-0XX + Stability towards photobleaching and pH
Chromeo546
  • Superior luminescence properties
  • Broad Stokes shift
  • Low cell toxicity
  • Soluble in DMSO, DMF, ethanol, MeCN
  • Chromeo™ is a trademark of Active Motif
  • Azide available for Click-Chemistry
  • Structure on the right: Chromeo™  546
Oyster® 555 552 572 150,000 5-0315-0XX - New class of cyanine dyes
Oyster
  • New class of cyanine dyes
  • High photostability
  • Bright fluorescence
  • Produced by Luminartis 
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Oyster® 555
Oyster® 550 551 570 150,000 5-0315-0XX + New class of cyanine dyes
Oyster
  • New class of cyanine dyes
  • High photostability
  • Bright fluorescence
  • Produced by Luminartis
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Oyster®
Atto® 550 554 576 120,000 5-0311-7XX + Highly photostable with high fluorescence yield
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 555 555 565 150,000 5-0316-0XX + Similar spectra to Cy3 but brighter and more photostable

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 555 can be 5', 3' or internally coupled to the oligo

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • Azide available for Click-Chemistry
  • fluorescence is stable from pH 4 to pH 10
Cyanine 3 550 570 150,000 5-0310-70X + Excellent spectroscopic properties, such as high quantum yields, high extinction coefficient and great stability of fluorescence signal against changes in pH, various buffers and other conditions. Azide available for Click-Chemistry.
Cy3TM

 

TAMRA, 6-isomer 547 573 91,000 5-0314-0XX + Used for in situ hybridization applications

Tetramethylrhodamine

  • Widely used in non-radioactive DNA sequencing
  • High photostability
  • Used for in situ hybridization applications
  • Used as a quencher in Fluorescence Resonance Energy Transfer applications (FRET)
  • Can be 5', 3' or internally coupled to the oligo
  • Available as 5- or 6-isomer
Alexa Fluor® 546 555 570 104,000 5-0316-0XX - Similar spectra to Cy3 but brighter and more photostable
AF546

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 546 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Structure on the right: Alexa Fluor® 546
Dy® 548 558 572 150,000 5-0311-7XX - Higher water solubility and polarity
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 547 557 574 150,000 5-0311-7XX + For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Very good alternative for Cy 3
  • Can be 5', 3' or internally coupled to the oligo
Dy® 560 559 578 120,000 5-0311-7XX + For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Dy® 550 562 577 120,000 5-0311-7XX - Soluble in water, methanol, DMF, DMSO
Dy550
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 550
Dy® 549P1 563 578 150,000 5-0311-7XX - Soluble in water and methanol
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® 565 563 592 120,000 5-0311-7XX + Highly photostable with high fluorescence yield
Atto565
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto 565
Atto® Rho3B 564 592 120,000 5-0311-7XX - Related to well-known dye Rhodamine B
  • Strong absorption
  • High thermal and photostability
  • Temperature dependent fluorescence yield
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
Atto® Rho11 571 595 120,000 5-0311-7XX - Very high fluorescence efficiency and exceptionally high photostability
  • New rhodamine dye
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
Carboxy-X-rhodamine (ROX), 6-isomer 575 602 82,000 5-0310-6XX + Used for non-radioactive sequencing
  • Widely used for non-radioactive DNA sequencing
  • Available as mixed, 5-and 6-isomer
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Atto® Rho12 576 601 120,000 5-0311-7XX - Strong absorption, high fluorescence quantum yield, and high photostability
  • New rhodamine dye
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
Carboxy-X-rhodamine (ROX), 5-isomer 574 602 78,000 5-0310-6XX + Used for non-radioactive sequencing
5ROX5isomer
  • Widely used for non-radioactive DNA sequencing
  • Available as mixed, 5-and 6-isomer
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-ROX, 5-isomer
Alexa Fluor® 568 575 560 91,000 5-0316-0XX - For confocal laser-scanning microscopy
AF568

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 568 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Structure on the right: Alexa Fluor® 568 succinimidyl ester
Eterneon™ Orange 570 Azide 573 612 50,000 5-0318-0XX + Azide suitable for Click-Chemistry
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Eterneon™ Orange 580 Azide 581 605 75,000 5-0318-0XX + Alternative dye for Cyanine 3.5
  • Alternative dye for Cyanine 3.5
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
BHQ-2 579 none 38,000 5-0317-1XX - Missing native fluorescence of the BHQ results in less background fluorescence
BHQ2

Black Hole QuencherTM dye

  • Robust dark quencher compatible with ammonium hydroxide deprotection
  • Excellent coupling efficiency
  • Non-fluorescent with large extinction coefficients
  • Also used for static quenching
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-BHQ-2 Phosphoramidite*

* Structure used with permission of Glen Research Corporation

Atto® Thio12 579 609 110,000 5-0311-7XX - Closely related to well-known rhodamines
AttoThio12
  • High yield of triplet formation
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® Thio12
Dy® 591 581 598 120,000 5-0311-7XX - Soluble in methanol, DMF, DMSO
Dy591
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 591
Dy® 590 580 599 120,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Abberior® STAR 580 583 605 64,300 5-0310-0XX - Ideal for STED microscopy
  • Soluble in methanol, acetonitrile, DMF, DMSO
  • Produced by Abberior
  • Can be 5', 3' or internally coupled to the oligo
Sulforhodamine 101 584 603 116,000 5-0314-4XX + The long wavelength emission property of Texas Red®-X is ideal for multicolor applications
Sulforhodamine101
  • The long wavelength emission property of Sulfohodamine 101 is ideal for multicolor applications
  • Extra long spacer between oligonucleotide and dye
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: 5'-Texas Red®-X, mixed isomers
Atto® 580Q 586 none 110,000 5-0311-7XX - Highly thermo and photo-stable
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
Atto® Rho101 586 610 120,000 5-0311-7XX - Extraordinary brightness and photostability
  • New rhodamine dye
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 594 590 615 73,000 5-0316-0XX + For multilabeling
AF594

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.)  Alexa Fluor® 594 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Azide available for Click-Chemistry
  • Structure on the right: Alexa Fluor® 594 carboxylic acid, succinimidyl ester
Cyanine 3.5 NHS 581 596 150,000 5-0310-7XX - For postsynthetic labeling
Cyanine 3.5 581 596 150,000 5-0310-71X - Excellent spectroscopic properties, such as high quantum yields, high extinction coefficient and great stability of fluorescence signal against changes in pH, various buffers and other conditions
Dy® 594 594 615 92,000 5-0311-7XX - Bright solid state emission
  • Suitable for protein labeling, microarray
    experiments, FisH microscopy, gel electrophoresis
  • Soluble in methanol, DMF, DMSO
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® 590 594 624 120,000 5-0311-7XX + Highly photostable with high fluorescence yield
Atto590
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® 590
Abberior® CAGE 590 586 607 43,800 5-0310-0XX - Photoactivatable dye
  • First CAGE dye in the red fluorescent regime
  • Photoactivatable: dye is initially colorless and nonfluorescent
  • When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Eterneon™ Red 600 Azide 593 624 50,000 5-0318-0XX + Azide suitable for Click-Chemistry
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Abberior® STAR 600 604 627 43,500 5-0310-0XX + Exceptionally bright red fluorescent dye
  • Exceptionally bright red fluorescent dye
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Dy® 601 XL 606 663 85,000 5-0311-7XX - Megastokes-Dye
  • Megastokes-Dye
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Abberior® CAGE 635 630 653 11,000 5-0310-0XX - Photoactivatable dye
  • First CAGE dye in the far red fluorescent regime
  • Photoactivatable: dye is initially colorless and nonfluorescent
  • When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
BBQ 650 598 none 40,667 5-0317-1XX + Excellent quencher of long-wavelength fluorescence in both FRET and contact modes
BBQ650
Black Berry Quencher
  • Excellent quencher of long-wavelength fluorescence in both FRET and contact modes
  • The absorbance between 550 and 750 nm allows efficient quenching of Cy3-Cy5.5
  • Missing native fluorescence of the BBQ results in less background fluorescence
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: BBQ
Dy® 605 600 624 110,000 5-0311-7XX - Hydrophilic dye
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® Rho13 600 625 120,000 5-0311-7XX - New rhodamine dye
  • Strong absorption
  • High fluorescence quantum yield
  • High thermal and photostability
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
Atto® 594 601 627 120,000 5-0311-7XX + Highly photostable with high fluorescence yield
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 610-X 602 524 132,000 5-0316-0XX - Longest-wavelength Alexa Fluor® dye with fluorescence that is visible to the human eye
AF610-X

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 610-X can be 5', 3' or internally coupled to the oligo. Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
  • Structure on the right: Alexa Fluor® 610-X, succinimidyl ester
Dy® 610 610 630 80,000 5-0311-7XX - Bright solid state emission
Dy610
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH 
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 610
Atto® 610 615 634 150,000 5-0311-7XX - Highly photostable with high fluorescence yield
Atto610
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Very base-sensitive, additional purification strongly recommended
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® 610
Atto® 612Q 615 none 114,000 5-0311-7XX - Highly photostable with high fluorescence yield
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
Atto® 620 619 643 120,000 5-0311-7XX - Temperature dependent fluorescence yield, highly photostable
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
Dy® 615 621 641 200,000 5-0311-7XX - Emission from DY-590 to DY-634
Dy615
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH  
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 615
Atto® Rho14 625 646 140,000 5-0311-7XX - Brightest label available in this wavelength range
  • Strong absorption
  • Extraordinarily high fluorescence quantum yield
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
Eterneon™ Red 630 Azide 629 646 113,000 5-0318-0XX + Azide suitable for Click-Chemistry
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Atto® 633 629 657 130,000 5-0311-7XX + Highly thermo and photo-stable with high fluorescence yield
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 633 632 647 239,000 5-0316-0XX - Bright and photostable, important for fluorescence imaging due to spectra being beyond the range of most sample autofluorescence.

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 633 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
Abberior® STAR 635 634 654 63,000 5-0310-0XX + Latest dye development for STED imaging in the red spectral region
  • Exceptionally bright and stable red fluorescent dye
  • High water solubility
  • Produced by Abberior
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Abberior® STAR 635P 634 654 80,000 5-0310-0XX + First phosphorylated dye
  • Very bright and very stable dye
  • Enables basically background free imaging
  • Produced by Abberior
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Dy® 634 635 658 200,000 5-0311-7XX - Higher water solubility and polarity
Dy634
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 634
Dy® 632 637 657 200,000 5-0311-7XX - Good water solubility, for microarray experiments, FISH microscopy, gel electrophoresis
Dy632
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 632
Dy® 631 637 658 200,000 5-0311-7XX - Good water solubility, for microarray experiments, FISH microscopy, gel electrophoresis
Dy631
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 631
Dy® 630 636 657 200,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy630
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 630
Dy® 633 637 657 200,000 5-0311-7XX - Good water solubility, for microarray experiments, FISH microscopy, gel electrophoresis
Dy633
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 633
Dy®Q 2 641 none 90,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® 647-N 644 669 150,000 5-0311-7XX + For single-molecule applications and high-resolution microscopy
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Very good alternative for Cy 5
  • Produced by ATTO-Tec 
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Dy® 647P1 653 672 25,000 5-0311-7XX + Enhanced water solubility and polarity
  • Enhanced water solubility and polarity
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 648P1 653 672 25,000 5-0311-7XX - Alternative dye for Cyanine 5
  • Pentamethine based fluorophore
  • Bright solid state emission
  • Spectral similar to Cyanine 5 and Alexa Fluor® 647
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Atto® 647 645 669 120,000 5-0311-7XX + Belongs to a new generation of fluorescent labels for the red spectral region
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Very little triplet formation
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Cyanine 5 NHS 649 670 250,000 5-0310-7XX - Water soluble, used in enzymatic methods and for FRET
Cyanine 5 649 670 250,000 5-0310-82X + One of the best dyes absorbing in the red spectra
Cy5
  • Azide available for Click-Chemistry
Chromeo™ 642 642 660 180,000 5-0310-0XX + Stability towards photobleaching and pH
Chromeo642
  • Superior luminescence properties
  • Broad Stokes shift
  • Low cell toxicity
  • Soluble in methanol, ethanol, DMSO, DMF
  • Chromeo™ is a trademark of Active Motif
  • Azide available for Click-Chemistry
  • Structure on the right: Chromeo™  642
Dy® 636 647 671 200,000 5-0311-7XX + Good water solubility, for microarray experiments, FISH microscopy, gel electrophoresis
Dy636
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy® 636
Abberior® STAR RED 638 655 212,000 5-0310-0XX - Exceptionally bright red fluorescent dye
  • Key feature of the dye is its comparably long lifetime of ~3.5ns
  • Alternative dye for ATTO® 647N, AlexaFluor® 647, or Cyanine 5
  • Can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Abberior GmbH
Oyster® 647 651 671 240,000 5-0315-0XX - New class of cyanine dyes
Oyster
  • New class of cyanine dyes
  • High photostability
  • Bright fluorescence
  • Produced by Luminartis 
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Oyster® 647
Dy® 635 647 671 200,000 5-0311-7XX + For microarray experiments, FISH microscopy, gel electrophoresis
Dy635
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'- Dy® 635
Eterneon™ Red 645 Azide 643 662 25,000 5-0318-0XX + Alternative dye for Cyanine 5
  • Bright fluorescent dye
  • Alternative dye for Cyanine 5
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Alexa Fluor® 647 650 665 239,000 5-0316-0XX + Identical spectra but significantly higher fluorescence than the Cy5 dye

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 647 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • Azide available for Click-Chemistry
  • fluorescence is stable from pH 4 to pH 10
Oyster® 650 651 671 240,000 5-0315-0XX + New class of cyanine dyes
Oyster
  • New class of cyanine dyes
  • High photostability
  • Bright fluorescence
  • Produced by Luminartis
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Oyster® 650
Dy® 647 653 672 250,000 5-0311-7XX + For microarray experiments, FISH microscopy, gel electrophoresis
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH  
  • Very good alternative for Cy 5
  • Can be 5', 3' or internally coupled to the oligo
Dy® 652 654 675 220,000 5-0311-7XX - Soluble in methanol, ethanol, DMF, DMSO
Dy652
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 652
Dy® 654 653 677 220,000 5-0311-7XX + Soluble in water, methanol, DMF
Dy654
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy 654
Dy® 649P1 654 672 250,000 5-0311-7XX - Alternative to Dy 649
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 648 653 674 250,000 5-0311-7XX + Good water solubility
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 651 656 678 220,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy651
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 651
Dy® 650 653 674 220,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy650
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 650
Dy® 649 656 670 250,000 5-0311-7XX + Soluble in water and methanol
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Atto® MB2 658 none 110,000 5-0311-7XX - Derivative of the well-known redox dye Methylene Blue
AttoMB2
  • Can be reversibly reduced to the colorless leukoform. Upon oxidation (e.g., with oxygen) the dye recovers, and the absorption is fully restored
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Atto® MB2
Dy®Q 660 660 none 140,000 5-0311-7XX - As donor compatible with DY-630
Dyq660
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy®Q 660
Dy®Q 661 662 none 140,000 5-0311-7XX - As donor compatible with DY-630
Dyq661
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy®Q 661
Atto® 655 663 684 125,000 5-0311-7XX + For single-molecule applications and high-resolution microscopy
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Atto® Oxa12 663 684 125,000 5-0311-7XX - Excellent solubility in polar organic solvents like DMF, DMSO or acetonitrile and outstanding ozone resistance
  • Lipophilic variety of Atto® 655
  • Strong absorption
  • Good fluorescence quantum yield
  • Excellent thermal and photostability
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
Atto® 665 674 694 180,000 5-0311-7XX + Highly suitable for single-molecule detection applications and high-resolution microscopy
  • Strong absorption
  • Extraordinarily high fluorescence quantum yield
  • High thermal and photostability
  • Exceptionally high stability towards atmospheric ozone
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 660 660 690 132,000 5-0316-0XX - Bright near-infrared fluorescence, used in flow cytometry as a second label for use with allophycocyanin (APC) conjugates

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 660 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
Methylene Blue (on request) 665 690 93,000 xxx - Highly thermo and photo-stable
MB2
  • Highly thermo and photo-stable
  • Redox Label
  • Cationic dye with moderate hydrophilicity
  • Produced by ATTO-Tec 
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: ATTO MB2
BHQ-3 672 none 42,700 5-3017-14X + Multiplex two, three and four dye combinations in one reaction tube without cross-talk between channels
BHQ3

Black Hole QuencherTM dye

  • Robust dark quencher compatible with ammonium hydroxide deprotection
  • Excellent coupling efficiency, 
  • Non-fluorescent with large extinction coefficients 
  • Also used for static quenching
  • Azide available for Click-Chemistry
  • Can be 5' or 3' coupled to the oligo
  • Structure on the right: 5'-BHQ-3 Phosphoramidite*

* Structure used with permission of Glen Research Corporation

Cyanine 5.5 NHS 675 694 250,000 5-0310-7XX - Water soluble, used in enzymatic methods and for FRET
Cyanine 5.5 Amidite 675 694 250,000 5-0310-9XX + Absorption in the far red with high quantum yields, a high extinction coefficient and great stability in various buffers and conditions
Cy5.5TM
Dy® 677 673 694 180,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy677
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 677
Dy® 678 674 694 180,000 5-0311-7XX - Very hydrophilic
Dy678
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 678
Dy® 675 675 699 180,000 5-0311-7XX - Suitable for microarray experiments, FisH microscopy, gel electrophoresis
Dy675
  • Bright solid state emission
  • Soluble in methanol, ethanol, DMF, DMSO
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 675
Dy® 676 674 699 180,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy676
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 676
Oyster® 680 674 693 250,000 5-0315-0XX - New class of cyanine dyes
Oyster
  • New class of cyanine dyes
  • High photostability
  • Bright fluorescence
  • Produced by Luminartis
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Oyster® 680
Eterneon™ Far Red 680 Azide 677 701 195,000 5-0318-0XX + Alternative dye for Cyanine 5.5
  • Alternative dye for Cyanine 5.5
  • Azide suitable for Click-Chemistry
  • Product of baseclick GmbH
Dy® 679P1 679 697 200,000 5-0311-7XX - Alternative to Dy 679
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Alexa Fluor® 680 680 700 184,000 5-0316-0XX - Ideal for three- and four-color labeling

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 680 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
Atto® 680 680 700 125,000 5-0311-7XX + Highly thermo and photo-stable with high fluorescence yield
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Dy®Q 3 683 none 80,000 5-0311-7XX - Soluble in water and methanol
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 680 690 709 140,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy680
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 680
Dy® 682 690 709 140,000 5-0311-7XX + Goodwater solubility and polarity
Dy682
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy® 682
Dy® 681 691 708 140,000 5-0311-7XX + For microarray experiments, FISH microscopy, gel electrophoresis
Dy682
  • MegaStokes Dyes (XL)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy® 681
DyQ 700 696 none 48,500 5-0311-7XX - Polar water soluble quencher
  • Produced by Dyomics
  • Can be 5', 3' or internally coupled to the oligo
Atto® 700 700 718 120,000 5-0311-7XX + Highly thermo and photo-stable with high fluorescence yield
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
Alexa Fluor® 700 702 723 192,000 5-0316-0XX - Near-infrared fluorescence emission

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 700 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
Dy® 703 705 721 140,000 5-0311-7XX - Soluble in water, methanol, ethanol, DMF, DMSO
Dy703
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 703
Dy® 704 706 721 140,000 5-0311-7XX - Very hydrophilic
Dy704
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right
Dy® 700 707 730 140,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy700
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy-700 to Dy-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 700
Dy® 701 706 731 140,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy701
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 701
Atto® 725 725 752 120,000 5-0311-7XX + Highly photostable
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec 
  • Very base-sensitive, additional purification strongly recommended
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
Dy® 734 734 766 240,000 5-0311-7XX + Good water solubility and polarity
Dy734
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy-700 to Dy-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: Dy® 734
Dy® 730 732 758 240,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy730
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 730
Dy® 732 736 759 240,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy732
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 732
Dy® 731 736 760 240,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy731
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 731
Atto® 740 740 764 120,000 5-0311-7XX + Highly photostable
  • Strong absorption
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications
  • Produced by ATTO-Tec
  • Very base-sensitive, additional purification strongly recommended
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Dy® 754 748 771 270,000 5-0311-7XX - Soluble in water, methanol, DMF
Dy754
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 754
Alexa Fluor® 750 749 775 240,000 5-0316-0XX - Good for multicolor analysis

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.)  Alexa Fluor® 750 can be 5', 3' or internally coupled to the oligo.

Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
Dy® 752 748 772 270,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy752
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 752
Cyanine 7 NHS 748 767 250,000 5-0310-7XX - Water soluble, used in enzymatic methods and for FRET
Dy® 750 747 776 270,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy750
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 750
Dy® 751 751 779 270,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy751
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 751
Dy® 749P1 759 780 240,000 5-0311-7XX + Very hydrophilic
  • Near Infrared (Dy® 700 to Dy® 831)
  • Produced by Dyomics GmbH
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
Dy®Q 4 766 none 180,000 5-0311-7XX - Soluble in water and methanol
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
Dy® 778 767 787 240,000 5-0311-7XX - Very hydrophilic dye
Dy778
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 778
Dy® 777 770 788 240,000 5-0311-7XX - Hydrophilic dye
Dy777
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 777
Dy® 776 771 801 240,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy776
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 776
Dy® 780 782 800 170,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy780
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 780
Dy® 781 783 800 170,000 5-0311-7XX - For microarray experiments, FISH microscopy, gel electrophoresis
Dy781
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 781
Dy® 782 (infrared!) 782 800 102,000 5-0311-7XX + Good water solubility and polarity
Dy782
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Azide available for Click-Chemistry
  • Structure on the right: 5'-Dy® 782
Alexa Fluor® 790 784 814 260,000 5-0316-0XX - Brighter fluorescence and photostability than others

We have obtained a world-wide license to label oligonucleotides (DNA and RNA) with Alexa Fluor® dyes from Molecular Probes (owned by Invitrogen Corp.) Alexa Fluor® 790 can be 5', 3' or internally coupled to the oligo
Oligonucleotides labeled with Alexa Fluor have many advantages:

  • brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores
  • potentially better results of measurements in e.g. Fluorescence Correlation Spectroscopy (FCS) than with conventional fluorophores
  • fluorescence is stable from pH 4 to pH 10
Dy® 800 777 791 280,000 5-0311-7XX + Hydrophilic
  • Hydrophilic
  • Azide available for Click-Chemistry
  • Can be 5', 3' or internally coupled to the oligo
  • Product of Dyomics GmbH 
Dy® 831 844 none 220,000 5-0311-7XX - Negatively charged
Dy831
  • MegaStokes Dyes (XL)
  • Near Infrared (Dy®-700 to Dy®-831)
  • Produced by Dyomics GmbH
  • Can be 5', 3' or internally coupled to the oligo
  • Structure on the right: Dy® 831