Quality of dye labeled oligonucleotides

  • Double HPLC purification included
  • Excessive dye removed
  • Unlabeled oligonucleotides removed

The delivery amount is 5 nmol* with the 0.05 µmol scale, 25 nmol* with the 0.2 µmol scale, and 100 nmol* for the 1.0 µmol scale.

All dye labeled oligonucleotides are double HPLC purified (with the exception of the directly coupled dyes where no excessive dye must be eliminated). Failure sequences are removed during the first HPLC purification. The second HPLC is performed to remove unlabeled oligonucleotides and excessive dye. As a result only labeled oligonucleotides are obtained.

* for oligonucleotides shorter 40 bases