pASK-IBA5C

Contents: 5 µg Cat.no: 2-1324-000 Price: USD 202.00  

The pASK-IBA5C vector allows the expression of Strep-tag®-fusion-proteins in E.coli.

The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the Strep-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any E. coli strain because the tet-promoter works independently from the genetic background of E.coli.

  • promoter from bp 37 to 72
  • forward primer binding site from bp 57 to 76
  • Strep-tag® from bp 139 to 171
  • multiple cloning site from bp 172 to 253
  • reverse primer binding site from bp 321 to 337
  • f1 origin from bp 337 to 775
  • CamR resistance gene from bp 910 to 1569
  • tet-repressor from bp 1582 to 2205