pASK-IBA2

Contents: 5 µg Cat.no: 2-1301-000 Price: USD 202.00  

The pASK-IBA2 vector allows the expression of Strep-tag®-fusion-proteins in E.coli.

The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the Strep-tag® for C-terminal fusion to the recombinant protein and the Ampicilin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli.

  • Promoter from bp 37 to 72
  • forward primer binding site from bp 57 to 76
  • OmpA signal sequence from bp 139 to 201
  • multiple cloning site from bp 202 to 282
  • Strep-tag® from bp 283 to 312
  • reverse primer binding site from bp 368 to 384
  • f1 origin from bp 397 to 835
  • AmpR resistance gene from bp 984 to 1844
  • tet-repressor from bp 1854 to 2477