Root > Applications > Protein Production & Assays > Detection > IF/FACS > Immunofluorescence and FACS analysis of Strep-tagged proteins

Immunofluorescence

Strep-tag-FasL fusion protein stained with StrepMAB Classic Oyster 556 1:200 in MF7 cells. Fixation: 4%Formaldehyd, 4°C 20min Kindly provided by: Dr. U. Bertsch, Inst. f. Immunologie, UK Kiel

 

Strep-NogoB fusion protein stained with StrepMAB Classic Oyster 488 1:300 in Schwann cells. Fixation: 100% MetOH 10 min RT StrepMAB Classic 488 1:300 in 3% NGS/1% BSA 4°C o/n Kindly provided by: Dr. rer. nat. Rüdiger Schweigreiter, Sektion für Neurobiochemie, Biozentrum, Medizinische Universität Innsbruck Type-I transmembrane protein (extracellular domain N-terminal Strep-tagged) stained with Strep-Tactin Chromeo 546 1:100 in insect cells. Fixation: 4% Formaldehyd in PBS, 20minuten RT Kindly provided by: Dr. Michael Krahn, , Abteilung Stamm-zellbiologie, GZMB Göttingen

FACS

FACS analysis of Zymosan, stained with StrepMAB Immo Oyster 645 and - Chromeo 642 against Clec7-One-STrEP-tag 1:100, 20 min ice Kindly provided by : Dr. K. Neumann, III Med. Klinik, TUM München