References about detection

Strep-tag review

Schmidt, T.G.M. and Skerra, A. (2007)
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Nature Protocols 2, 1528-1535.

Tag comparison
  1. Vasilescu J. and Figeys D. (2006)
    Mapping protein–protein interactions by mass spectrometry. 
    Current Opinion in Biotechnology 17, 394–399.
  2. Lichty, J.J., Malecki, J.L., Agnew, H.D., Michelson-Horowitz, D.J. and Tan, S. (2005) 
    Comparison of affinity tags for protein purification. 
    Prot. Expr. Purific. 41, 98-105.
  3. Cass, B., Pham, P.L., Kamen, A. and Durocher, Y. (2004) (double-tag, Strep-His-tag)
    Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. 
    Prot. Expr. Purific. 40, 77-85.
  4. Witte, C.P., Noël, L.D., Gielbert, J., Parker, J.E. and Romeis, T. (2004) (Plant expression)
    Rapid one-step protein purification from plant material using the eight-amino acid strepII epitope. 
    Plant Molec. Biol. 55, 135-147.
  5. Terpe, K. (2003) 
    Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. 
    Appl. Microbiol. Biotechnol. 60, 523-53.
Applications

Detection

  1. Müller I. B., Knöckel J., Groves M. R., Jordanova R., Ealick S. E., Walter R.D., Wrenger C. (2008)
    The Assembly of the Plasmodial PLP Synthase Complex Follows a Defined Course. 
    PlosOne 3 (3), e1815.
  2. Ichioka F., Kobayashi R., Katoh K., Shibata H. and Maki M. (2008)
    Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4). 
    FEBS Journal 275 (4), 682-692.
  3. Kurdyukov S., Faust A., Nawrath C., Bär S., Voisin D., Efremova N., Franke R., Schreiber L., Saedler H., Métraux J.-P., and Yephremova A. (2006)
    The Epidermis-Specific Extracellular BODYGUARD Controls Cuticle Development and Morphogenesis in Arabidopsis. 
    The Plant Cell 18, 321–339.
  4. Rummel, A., Mahrhold, S., Bigalke H. and Binz, T. (2003) 
    The HCC-domain of botulinum neurotoxins A and B exhibits a singular ganglioside binding site displaying serotype specific carbohydrate interaction. 
    Molec. Microbiology 51, 631-643
  5. Ribrioux, S., Kleymann, G., Haase, W., Heitmann, K., Ostermeier, C. and Michel, H. (1996) 
    Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunochemistry. 
    The Journal of Histochemistry and Cytochemistry 44, 207-213.
  6. Kleymann, G., Iwata, S., Wiesmüller, H.H., Ludwig, B. and Michel, H. (1995) 
    Immunoelectronic microscopy and epitope mapping with monoclonal antibodies suggests the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases. 
    Eur. J. Biochem. 230, 359-363.
  7. Kleymann, G., Ostermeier, C., Heitmann, K., Haase, W. and Michel H. (1995) 
    Use of antibody fragments (Fv) in immunochemistry. 
    The Journal of Histochemistry and Cytochemistry 43, 607-614.
  8. Tsiotis, G., Haase, W., Engel, A. and Michel, H. (1995) 
    Isolation and structural characterization of trimeric cyanobacterial photosystem I complex with the help of recombinant antibody fragments. 
    Eur. J. Biochem. 231, 823-830.
Assays/HTS
  1. Ernst, W.J., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R.M. (2000) 
    Expanding baculovirus surface display modification of the native coat protein Gp64 of autographa californica NPV. 
    Eur. J. Biochem. 267, 4033-4039.
  2. Panke, O., Gumbiowski, K., Junge, W. and Engelbrecht, S. (2000) 
    F-ATPase: specific observation of the rotating c subunit oligomer of EFoEF1. 
    FEBS Letters 472, 34-38.
  3. Skerra, A. and Schmidt, T.G.M. (1993) 
    Use of the strep-tag and streptavidin for detection and purification of recombinant proteins. 
    Methods in Enzymology 326, 271-304.
  4. Sebastian, P., Wallwitz, J. and Schmidt, S. (2003) 
    Semi automated production of a set of different recombinant GST-streptag fusion proteins.
    J. Chromatogr. B 786, 343-355