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Strep-Tactin®

Specifications of Strep-Tactin® HRP conjugate
Detection
N- or C-terminal or internal Strep-tag® II
Sensitivity in dot blots
> 7 pg/dot
Sensitivity in Western blots 
> 7 pg/band
Cross reactivity  
Reacts with biotin carboxyl carrier protein (22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with Biotin Blocking Buffer.
Form
Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01% Thimerosal as preservative. Can be diluted 1:10,000 for detection of Strep-tag® II fusion proteins bound to microplates or 1:4,000 for detection of Strep-tag® II fusion proteins after Western blotting.
Stability
1 year
Storage
4 °C
Shipment
RT

 

Specifications of Strep-Tactin® AP conjugate
Alkaline Phosphatase Calf intestine
Detection N- or C-terminal or internal Strep-tag® II
Sensitivity in dot blots  > 1 ng/dot
Sensitivity in Western blots
> 2 ng/band
Cross reactivity  
Reacts with Biotin Carboxyl Carrier Protein (BCCP, 22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with 2 µg/ml Biotin Blocking Buffer.
Form Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01 % Thimerosal as preservative. Can be diluted 1:4,000 for detection of Strep-tag® II fusion proteins after Western blotting. (Please note that for ELISA we recommend to use the Strep-Tactin® HRP conjugate)
Stability 1 year
Storage 4 °C
Shipment RT

Monoclonal antibodies

Specifications StrepMAB®-Classic, 2-1507-001
Purification
purified by affinity chromatography
Host animal/Subclass
Mouse/IgG1
Specificity
N- or C-terminal or internal Strep-tag® II
Form
lyophilized from NH4HCO3 with 10 mg sucrose per mg antibody
Stability
18 months after shipment
Storage
stored at -20 °C
Shipment
RT
Specifications of StrepMAB®-Classic HRP conjugate, 2-1509-001
Purification
purified by affinity chromatography
HRP conjugate
conjugated to 3-3.5 mols horseradish peroxidase per mol IgG
Host animal/Subclass
Mouse/IgG1
Specificity
N- or C-terminal or internal Strep-tag® II
Form
suspension in PBS with 0.5 % BSA and 0.01 % thimerosal
Stability
18 months after shipment
Storage
stored at 4 °C, do not freeze!
Shipment
RT
Specifications of Rabbit anti Mouse pAb, HRP conjugated, 2-1591-001
Description
Purified polyclonal rabbit anti mouse immunoglobulin fraction conjugated to horseradish peroxidase (HRP).
Specifity
The antibody used for conjugation has not been absorbed to remove cross-reactions to immunoglobulin from other species. Therefore the conjugate shows reactions with mouse immunoglobulin of all classes
Form
Dissolved in 50 mM Tris/HCl 15 mM NaN3, pH 7.2
Concentration of conjugate
1.3 mg/ml
Amount
0.5 ml
Dilution
  • Dilute 1:1000 for chromogenic detection of bound mouse monoclonal antibodies in Western Blots
  • Dilute 1:2000 (or titrate up to 1:10,000) for detection of mouse monoclonal antibodies in microplate assays
Storage
2 - 8 °C
Stability
6 months after shipping
Shipment
RT