Streptamer® technology for fluorescent cell staining & magnetic cell isolation
Uncompromised, authentic cells for research and diagnostics - watch video (in German)
The Streptamer® technology allows both fluorescent staining as well as magnetic isolation of cells with reversible reagents. Virtually all eukaryotic cells can be selected with this technology. The reversible Streptamers® not only show excellent staining intensities, but they can be removed from the cells to optimally preserve cell effector function, making it a versatile platform, not only for basic research, but also for safe and efficient diagnostic use.
are designed for the selection of lymphocytes carrying a surface marker which defines a cell subset, e.g. T cells (CD3, CD4, CD8), memory T cells (CD8, CD62L, CD45RA-) and regulatory T cells (CD4, CD25, CD45RA). Magnetic isolation is performed with microbeads and a hand-held magnet. For fluorescent staining, Strep-Tactin PE or APC for Fab Streptamers is offered.
are designed for the selection of antigen-specific CD8+ T cells. Magnetic isolation is performed with nanobeads and magnetic columns. For fluorescent staining, Strep-Tactin PE or APC for MHC I Streptamers® is offered.
Magnetic cell isolation (Fab Streptamers®):
Fluorescent cell staining (MHC I Streptamers®):
The Streptamer® principle: full reversibility
The Streptamer® reagents: a modular system
Fab-Streps or MHC-Streps are either combined with fluorescence- or bead-labeled Strep-Tactin® for cell staining or magnetic isolation, respectively. Biotin is offered as stock solution with the Streptamer® Solution Set which is used for removal of cell staining as well as cell isolation reagents. This modular reagent system enables the economic generation of multiple receptor- or antigen-specific staining and isolation reagents. All reagents can be purchased as single products or assembled in ready-to-use staining and isolation kits.