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Magnetic MHC I Streptamers® for antigen-specific isolation of (CD8+) T cells with reversible reagents

Magnetic MHC I Streptamers® comprise a MHC I-Strep which has to be associated with Strep-Tactin® Magnetic Nanobeads in a short incubation step. The resulting Streptamers® are then used for antigen-specific isolation of the target cells on magnetic columns. After isolation, the Streptamers® can be completely removed from the cells with biotin (stock solution included in the Streptamer® Solution Set Standard for washing and dissociation).

Modular Streptamer® technology: The MHC I-Streps can be associated either with a Strep-Tactin® fluorochrome for cell staining or with a Strep-Tactin® Magnetic Bead for cell isolation.

Single MHC I Streptamer® products for magnetic cell isolation
Component
Use
Size
choose among a great variety
of different alleles and antigens
for staining of 2.5x10^8 cells or for isolation out of 5x10^8 cells human or 2.5x10^8 cells mouse*
to be associated with MHC I-Streps
for isolation out of 5x10^8 cells human or 2.5x10^8 cells mouse*
Buffer IS 10x for washing
Biotin stock solution for dissociation
1 x 50 ml
1 x 1 ml
Buffer IS 10x for washing
Biotin stock solution for dissociation
1 x 550 ml
1 x 5 ml
5) Nylon filter tubes for removal of cell clumps 10 pieces

*For larger amounts please refer to the product list or inquire at info@streptamer.com

MHC I Streptamer® Magnetic Isolation Kits

Assemble your MHC I Streptamer® magnetic Isolation Kit in two steps:

  1. Choose your desired Isolation Kit; use filters "Category" and "Subcategory" on top of the product list
  2. Select your preferred MHC I-Strep

The kit includes magnetic nanobeads, MHC I-Streps and Solution Set Standard (Buffer IS for washing and Biotin for dissociation) for isolation out of 5x10^8 cells human or 2.5x10^8 cells mouse.

Streptamer® Custom Service

We develop and produce MHC I Streptamers with your specific allele and antigen, please refer to our Custom service.

Please contact info@streptamer.com for further information.