StarGate® for fast protein expression cloning

StarGate® has been developed for the rapid systematic screen of the optimal expression (regarding expression host and/or promoter) and purification (regarding the fusion tag) system for a given gene of interest (GOI).

There are two possible cloning procedures:

Direct Transfer Cloning

The direct transfer cloning procedure is recommended for proteins with known purification conditions. With this strategy the PCR product of a certain GOI can directly be transferred within a one-step-reaction into the appropriate Acceptor Vector.

“Two-step-cloning” via pENTRY-IBA

StarGate® cloning via the pENTRY-IBA vector provides a tool for the systematic and rapid screen of the best working expression conditions for a certain GOI, where nothing is known from literature. After initial pENTRY cloning, the GOI in the resulting Donor Vector can be easily transferred by a simple one-tube reaction into a multitude of Acceptor Vectors providing different features.

Acceptor Vectors

IBA Acceptor Vectors provide different genetic surroundings for optimal protein expression and purification. These include different purification tags, promoter for a variety of host cells, signal sequences etc.

For an on overview on the entire vector portfolio please, click the Acceptor Vector Table.