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Protein:Protein Interaction (PPI) Analysis

StarGate® for Bait Cloning

The StarGate cloning system is also a perfect tool for efficient screening and fast identification of the optimal tag for protein:protein interaction (PPI) investigation with a given bait. Once the bait protein is cloned into the Donor Vector, a large selection of Acceptor Vectors for its expression with different tag arrangements is available.

For PPI analysis we provide different Acceptor Vectors to generate the desired bait expression vector.

 

For the different PPI analysis methods (click here for more details) we provide Acceptor Vectors equipped with the following features:

 

Twin-Strep
Vectors with Twin-Strep-tag® for N- and C-terminal fusion
(available for E. coli and mammalia)
One-TAP
Vectors with Twin-Strep-tag® for N- and C-terminal fusion
(available for E. coli and mammalia)
Two-TAP
Vectors with Twin-Strep / FLAG®-tag double tag for N- and C-terminal fusion
(available for E. coli and mammalia)
SPINE
Vectors with Strep-tag®II at N- and C-terminus
(available for E. coli)