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Specifications

StrepMAB-Classic

StrepMAB-Classic, Cat. No. 2-1507-001

Purification purified by affinity chromatography 
Host animal/Subclass  Mouse/IgG1
Specificity  N- or C-terminal or internal Strep-tag® II
Form  lyophilized from NH4HCO3 with 10 mg sucrose per mg antibod
Stability  18 months after shipment 
Storage  stored at -20 °C 
Shipment  RT
 
StrepMAB-Classic, HRP conjugate

StrepMAB-Classic, HRP conjugate, Cat. No. 2-1509-001

Purification
purified by affinity chromatography
HRP conjugate
conjugated to 3-3.5 mols horseradish peroxidase per mol IgG
Host animal/Subclass
Mouse/IgG1
Specificity
N- or C-terminal or internal Strep-tag® II
Form
suspension in PBS with 0.5 % BSA and 0.01 % thimerosal
Stability
18 months after shipment
Storage
stored at 4 °C, do not freeze!
Shipment
RT
 
StrepMAB-Immo

StrepMAB-Immo, Cat. No. 2-1521-001

Host animal/Subclass
Mouse/IgG1
Specifity
Nearly irreversible binding of C- or N-terminally fused Strep-tag® II, which is N-terminally extended by "SerAla" ** like recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein. Proteins containing Strep-tag® II with
N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far.
Form
binding capacity
lyophilized from NH4HCO3 with 10 mg sucrose per mg antibody
(determined for bacterial alkaline phosphatase)
Stability
24 months after shipment
Storage
-20 °C
Shipment
RT
**pASK-/pEXPR- and pPR-IBA vectors with N-term One-/Strep-tag® II do NOT have "SerAla"-Linker but the StarGate® vectors (carrying Strep-tag® II and One-STrEP-tag) are equipped with the SA-Linker.
Secondary antibody, HRP conjugate

Rabbit anti Mouse pAb, HRP conjugate Cat. No. 2-1591-001

Description
Purified polyclonal rabbit anti mouse immunoglobulin fraction conjugated to horseradish peroxidase (HRP).
Specifity
The antibody used for conjugation has not been absorbed to remove cross-reactions to immunoglobulin from other species. Therefore the conjugate shows reactions with mouse immunoglobulin of all classes
Form
Dissolved in 50 mM Tris/HCl 15 mM NaN3, pH 7.2
Concentration of conjugate
1.3 mg/ml
Amount
0.5 ml
Dilution
  • Dilute 1:1000 for chromogenic detection of bound mouse monoclonal antibodies in Western Blots
  • Dilute 1:2000 (or titrate up to 1:10,000) for detection of mouse monoclonal antibodies in microplate assays
Storage
2 - 8 °C
Stability
6 months after shipping
Shipment
RT