Root > Products > Strep-Tactin® & Strep-tag® > Immobilization Plates > Strep-Tactin Coated Microplates and StrepMAB Immo for Strep-tag Protein Immobilization

Strep-tag®-protein immobilization

Immobilization of proteins can be useful for the following applications:

  • Antibody or serum screening
  • Diagnostic assays
  • Expression cloning
  • Protein interaction studies
  • Screening of engineered enzymes
  • Drug screening

IBA products

Strep-Tactin XT coated microplates for (e)xtra tight immobilization of Twin-Strep-tag® fusion proteins

- eXtra Tight (pM affinity) immobilization of Twin-Strep-tag® fusion proteins

Ready-to-use microplates (12 x 8-well strips) for diagnostic/assay applications, coated with Strep-Tactin® XT, a new Strep-Tactin® variant with higher affinity for Strep-tag® II fusion proteins (nM) and especially Twin-Strep-tag® fusion proteins (pM). They are selectively captured from complex mixtures and the biomolecules of interest are presented to interacting partners in a uniform manner which results in reliable and reproducible assays. The solid-phase, multi-well format for convenient assays and high throughput screenings is compatible with standard multichannel pipettes and automated plate washers and plate readers.

Note that the bound fusion protein can still be eluted from the microplate.

Strep-Tactin® coated microplates

Strep-Tactin® coated microplates

Fast, selective and sensitive assay for 8 to 96 samples

Strep-tactin coated 8-well strips and 96-well plates
Microplate consisting of twelve 8-well strips
coated with Strep-Tactin®

Ready-to-use Strep-Tactin® coated 8-well strips provide the power of our Strep-tag® system in a solid-phase, multi-well format for convenient assays and high throughput screenings of biomolecules tagged with Strep-tag® II. The strips are supplied framed in sets of 12, resulting in a 96-well configuration compatible with standard multichannel pipettes and automated plate washers and plate readers. The biomolecules are presented to interacting partners in a uniform manner, which results in reliable and reproducible assay formats.

StrepMAB Immo

StrepMAB Immo

High-affinity Strep-tag® II (Twin-Strep-tag) specific monoclonal antibody for capturing Strep-tag fusion proteins on solid phases

optimal for capturing Strep-tag fusion proteins on solid phases (e.g. Biacore chips1)
high affinity both with C- and N-terminal Strep-tag® fusion, provided the Strep-tag® is N-terminally extended by a SerAla-linker
for Western blots use StrepMAB-Classic HRP conjugate

1For more information on Biacore applications refer also to http://www.biacore.com/lifesciences/.

StrepMAB-Immo is a murine, high-affinity Strep-tag® II specific monoclonal antibody which is especially suited for stable, mild and oriented capturing of Strep-tag® II fusion proteins on solid phases. To realize this, the antibody can be immobilized on e.g. microplates, Biacore CM5 sensor chips or other biochips. The nearly irreversible binding is achieved both for fusion proteins carrying a C- or N- terminal Strep-tag®. The Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein). 

 

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for

  • Oriented immobilization of recombinant proteins with N-terminal, C-terminal or internal Strep-tag® II
  • Effective screening procedures
  • Minimal non-specific binding
  • High and constant activity
  • Minimal coefficients of variation (cv)