General:

Strep-tag® review

Schmidt, T.G.M. and Skerra, A. (2007)
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Nature Protocols 2, 1528-1535.

Tag comparison
  1. Vasilescu J. and Figeys D. (2006)
    Mapping protein–protein interactions by mass spectrometry. 
    Current Opinion in Biotechnology 17, 394–399.
  2. Lichty, J.J., Malecki, J.L., Agnew, H.D., Michelson-Horowitz, D.J. and Tan, S. (2005) 
    Comparison of affinity tags for protein purification. 
    Prot. Expr. Purific. 41, 98-105.
  3. Cass, B., Pham, P.L., Kamen, A. and Durocher, Y. (2004) (double-tag, Strep-His-tag)
    Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. 
    Prot. Expr. Purific. 40, 77-85.
  4. Witte, C.P., Noël, L.D., Gielbert, J., Parker, J.E. and Romeis, T. (2004) (Plant expression)
    Rapid one-step protein purification from plant material using the eight-amino acid strepII epitope. 
    Plant Molec. Biol. 55, 135-147.
  5. Terpe, K. (2003) 
    Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. 
    Appl. Microbiol. Biotechnol. 60, 523-53.
General Strep-tag® publications
  1. Müller, H.N. and Schmidt, T.G.M. (2000) 
    Simple and fast one-step purification of recom-binant proteins using the unique strep-tag technology. in Kastner M (Ed.)
    J. Chromatography. 61, 825-837.
  2. Skerra, A. and Schmidt, T.G.M. (2000) 
    Use of the strep-tag and streptavidin for detectionand purification of recombinant proteins. 
    Methods Enzymol. 326, 271-304.
  3. Schmidt, T.G.M. and Skerra, A. (2000) 
    Protein engineering for affinity purification: the strep-tag. in Alberghina L (Ed.) 
    Protein Engineering Industrial Biotechnology 41-61.
  4. Skerra, A. and Schmidt, T.G.M. (1999) 
    Applications of a peptide ligand for streptavidin:the strep-tag. 
    Biomolecular Engineering. 16, 79-86.

Expression hosts:

E. coli
  1. Ambrus A., Torocsik B., Adam-Vizi V. (2009)
    Periplasmic cold expression and one-step purification of human dihydrolipoamide dehydrogenase. 
    Protein Expression and Purification 63, 50-57.
  2. Müller I.B., Wu F., Bergmann B., Knöckel J., Walter R.D. (2009) 
    Poisoning Pyridoxal 5-Phosphate-Dependent Enzymes: A New Strategy to Target the Malaria Parasite Plasmodium falciparum. 
    PLoS ONE 4(2), e4406.
  3. Joseph R.E., Andreotti A.H. (2008) 
    Bacterial expression and purification of Interleukin-2 Tyrosine kinase: Single step separation of the chaperonin impurity. 
    Protein Expression and Purification 60 (2), 194-197.
  4. Möker N., Krämer J., Unden G., Krämer R., and Morbach S. (2007) 
    In Vitro Analysis of the Two-Component System MtrB-MtrA from Corynebacterium glutamicum
    Journal of Bacteriology 189 (9), 3645–3649.
  5. Han, R., Zwiefka, A., Caswell, C.C., Xu, Y., Keene, D.R., Lukomska E., Zhao, Z., Höök, M., Lukomski, S. (2006) 
    Assessment of prokaryotic collagen-like sequences derived from streptococcal Scl1 and Scl2 proteins as a source of recombinant GXY polymers. 
    App.l Microbiol. Biotechnol. 72, 109–115.
  6. Humtsoe, J.O., Kim, J.K., Xu, Y., Keene, D.R., Höök, M., Lukomski, S. and Wary, K.K. (2005) 
    A streptococcal collagen-like protein interacts with the integrin and induces intracellular signalling. 
    JBC 280, 13848-13857.
  7. Krug, A., Wendisch, V.F. and Bott, M. (2005)
    Identification of AcnR, a TetR-type repressor of the aconitase gene acnin Corynebacterium glutamicum. 
    JBC 280, 585-595.
  8. Xu, Y., Keene, D.R., Bujnicki, J.M., Höök, M. and Lukomski, S. (2002) 
    Streptococcal Scl1 and Scl2 proteins form collagen-like triple helices. 
    JBC 277, 27312-27318.
  9. Sundaram, M., Sivaprasadarao, A., van Aalten, D.M.F. and Findlay, J.B.C. (1998) 
    Expression, characterization and engineered specificity of rat epididymal retinoic acid-binding protein. 
    Biochem. J. 334, 155-160.
Mammalia
  1. G. Hassaine, C. Deluz, X.-D. Li, A. Graff, H. Vogel, H. Nury (2013)
    Large scale expression and purification of the mouse 5-HT3 receptor
    Biochimica et Biophysica Acta (BBA) - Biomembranes, http://dx.doi.org/10.1016/j.bbamem.2013.05.028.
  2. S. Bremer, K. Klein, A. Sedlmaier, M. Abouzied, V. Gieselmann and S. Franken (2013)
    Hepatoma-derived growth factor and nucleolin exist in the same ribonucleoprotein complex
    BMC Biochemistry, 14:2
  3. Ambepitiya Wickramasinghe I. N. , de Vries R. P. , Gröne A. , de Haan C. A. M. and Verheije M. H. (2011)
    Binding of Avian Coronavirus Spike Proteins to Host Factors Reflects Virus Tropism and Pathogenicity
    JOURNAL OF VIROLOGY, 8903–8912
  4. Ichioka F., Kobayashi R., Katoh K., Shibata H. and Maki M. (2008)
    Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4). 
    FEBS Journal 275 (4), 682-692.
  5. Johansen L.D., Naumanen T., Knudsen A., Westerlund N., Gromova I., Junttila M., Nielsen C., Bøttzauw T., Tolkovsky A., Westermarck J., Coffey E.T., Jäättelä M. and Kallunki T. (2008)
    IKAP localizes to membrane ruffles with filamin A and regulates actin cytoskeleton organization and cell migration. 
    Journal of Cell Science 121, 854-864.
  6. Gloeckner, C.J., Boldt, K., Schumacher, A., Roepman, R. and Ueffing, M. (2007) 
    A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes. 
    Proteomics 7, DOI 10.1002/pmic.200700038.
  7. Lichty, J.J., Malecki, J.L., Agnew, H.D., Michelson-Horowitz, D.J. and Tan, S. (2005) 
    Comparison of affinity tags for protein purification. 
    Prot. Expr. Purific. 41, 98-105.
  8. Cass, B., Pham, P.L., Kamen, A. and Durocher, Y. (2004) 
    Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. 
    Prot. Expr. Purific. 40, 77-85.
  9. Ahrens, T., Lambert, M., Pertz, O., Sasaki, T., Schulthess, T., Mège, R.M., Timpl, R. and Engel, J. (2003) 
    Homoassociation of VE-cadherin follows a mechanism common to classical cadherins. 
    J. Mol. Biol. 325, 733-742.
  10. Cotton, M., Stegmueller, K., Eickhoff, J., Hanke, M., Herzberger, K., Herget, T., Choidas, A.,Daub, H. and Godl, K. (2003) 
    Exploiting features of adenovirus replication to support mammalian kinase production. 
    Nucleic Acids Research 31, e128.
  11. Ahrens, T., Pertz, O., Häussinger, D., Fauser, C., Schulthess, T. and Engel, J. (2002) 
    Analysis of heterophilic and homophilic interactions of cadherins using the c-Jun/c-Fos dimerization domains. 
    JBC 277, 19455-19460.
  12. Sardy, M., Karpati, S., Merkl, B., Paulsson, M. and Smyth, N. (2002) 
    Epidermal transglutaminase (Tgase 3) is the autoantigen of dermatitis herpetiformis. 
    J. Exp. Med. 195, 747-757.
  13. Klatt, A. R., Nitsche, Nitsche, D.P., Kobbe, B., Macht, M., Paulsson, M. and Wagener, R. (2001) 
    Molecular structure, processing, and tissue distribution of matrilin-4. 
    JBC 276,17267-17275.
  14. Smyth, N., Odenthal U., Merkl B. and Paulsson, M. (2000) 
    Methods Eukaryotic expressionand purification of recombinant extracellular matrix proteins carrying the strep II tag. 
    Mol.Biol. 139, 49-57.
  15. Streuli, C.H. and Grant, M.E. (2000) 
    Extracellular matrix protocols.
    Methods Molec. Biol. 47-57.
  16. Sardy, M., Odenthal, U., Karpati, S., Paulsson, M. and Smyth, N. (1999) 
    Recombinanthuman tissue transglutaminase ELISA for the diagnosis of gluten-sensitive enteropathy. 
    Clinical Chem. 45, 2142-2149.
Insect cells
  1. Linnenbaum M., Busker M., Kraehling JR., Behrends S., (2012)
    Heme Oxygenase Isoforms Differ in Their Subcellular Trafficking during Hypoxia and Are
    Differentially Modulated by Cytochrome P450 Reductase.
    PLoS ONE 7(4): e35483. doi:10.1371/journal.pone.0035483
  2. Becker-Pauly C., Bruns B. C., Damm O., Schütte A., Hammouti K., Burmester  T. and Stöcker W. (2009) 
    News from an Ancient World: Two Novel Astacin Metalloproteases from the Horseshoe Crab. 
    J. Mol. Bio. 385,236-248.
  3. Iwasa M., Maeda K., Narita A., Maéda Y., and Oda T. (2008)
    Dual Roles of Gln137 of Actin Revealed by Recombinant Human Cardiac Muscle α-Actin Mutants. 
    JBC 283 (30), 21045-21053.
  4. Meyer T., Begitt A. and Vinkemeier U. (2007)
    Green fluorescent protein-tagging reduces the nucleocytoplasmic shuttling specifically of unphosphorylated STAT1. 
    FEBS Journal 274 (3), 815-826.
  5. Philomena Ostapchuk and Patrick Hearing (2008)
    Adenovirus IVa2 Protein Binds ATP
    Journal of Virology 82 (20), 10290–10294
Plants
  1. Dixon D. P., Hawkins T., Hussey P.J. and Edwards R. (2009)
    Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily
    Journal of Experimental Botany,60 (4), 1207–1218
  2. Kurdyukov S., Faust A., Nawrath C., Bär S., Voisin D., Efremova N., Franke R., Schreiber L., Saedler H., Métraux J.-P., and Yephremova A. (2006)
    The Epidermis-Specific Extracellular BODYGUARD Controls Cuticle Development and Morphogenesis in Arabidopsis. 
    The Plant Cell 18, 321–339.
  3. Witte, C.P., Noël, L.D., Gielbert, J., Parker, J.E. and Romeis, T. (2004) 
    Rapid one-step protein purification from plant material using the eight-amino acid strepII epitope. 
    Plant Molec. Biol. 55, 135-147.
  4. Drucker, M., German-Retana, S., Esperandieu, P., LeGall, O., and Blanc, S. (2002) 
    Purification of a viral protein form infected plant tissues using the strep-tag. 
    Bti BiotechInternational June Issue, 16-18.
  5. Wendt, U.K., Wenderoth, I., Tegeler, A. and von Schaewen, A. (2000) 
    Molecular characterization of a novel glucose-6-phosphate dehydrogenase from potato (Solanum Tuberosum L.). 
    The Plant J. 23, 723-733.
Cell-free expression

Schaffitzel C., Ban N. (2007)
Generation of ribosome nascent chain complexes for structural and functional studies.
Journal of Structural Biology 158, 463–471.

Lamla T., Hoerer S., Bauer M.M.T. (2006)
Screening for soluble expression constructs using cell-free protein synthesis
International Journal of Biological Macromolecules 39(1-3), 111-121.

Lamla, T., Stiege, W. and Erdmann, V.A. (2002)
An improved protein bioreactor. Efficient product isolation during in vitro protein biosynthesis via affinity tag. Molec.
Cellular Proteomics
1, 466-471.

Lamla, T., Mammeri, K. and Erdmann, V.A. (2001)
The cell-free protein biosynthesis-applications and analysis of the system.
Acta Biochimica Polonica 48, 453-465.

Further expression hosts

Schmidl, S.R., Hames, C. and Stülke, J. (2007)
Expression of Mycoplasma Proteins Carrying an Affinity Tag in M. pneumoniae Allows Rapid Purification and Circumvents Problems Related to the Aberrant Genetic Code.
Applied and Environmental Microbiology 73 (23), 7799–7801.

Protein-classes:

Protein complexes

Strep-tag

  1. Glatter T., Wepf A., Aebersold R. and Gstaiger M. (2009)
    An integrated workflow for charting the human interaction proteome: insights into the PP2A system. 
    Mol Syst Biol. 5: 237.
  2. Hernandez Alvarez, B., Hartmann, M. D., Albrecht, R., Lupas, A. N., Zeth, K. and Linke, D. (2008) 
    A new expression system for protein crystallization using trimeric coiled-coil adaptors. 
    Protein Engineering, Design & Selection 21 (1): 11–18.
  3. Merz, F., Boehringer, D., Schaffitzel, C., Preissler, S., Hoffmann, A., Maier, T., Rutkowska, A., Lozza, J., Ban, N., Bukau, B. and Deuerling, E. (2008) 
    Molecular mechanism and structure of Trigger Factor bound to the translating ribosome. 
    Embo J. 27, 1622-32.
  4. Thomae, A.W., Pich, D., Borcher, J, Spindler, M.-P., Berens, C, Hock, R., Hammerschmidt, W. and Schepers A. (2008) 
    Interaction between HMGA1a and the origin recognition complex creates site-specific replication origins. 
    PNAS 105 (5): 1692–1697.
  5. Wieland A., Denzel M., Schmidt E., Kochanek S., Kreppel F., Reimann J., Schirmbeck R. (2008)
    Recombinant complexes of antigen with stress proteins are potent CD8 T-cell-stimulating immunogens. 
    J Mol Med 86:1067–1079.
  6. Herzberg, C.,  Flórez Weidinger, L.A., Dörrbecker, B., Hübner, S., Stülke J. and Commichau, F.M. (2007) 
    SPINE: A method for the rapid detection and analysis of protein–protein interactions in vivo
    Proteomics. 7, DOI 10.1002/pmic.200700491.
  7. Larivière, L., Geiger, S., Hoeppner, S., Röther, S., Sträßer, K. and Cramer, P. (2006) 
    Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20. 
    Nature Structural and Molecular Biology 13 (10), 895-901.
  8. Sermwittayawong, D. and Tan, S. (2006) 
    SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment. 
    EMBO J. 25, 3791-80.
  9. Vasilescu, J. and Figeys, D. (2006) 
    Mapping protein-protein interactions by mass spectrometry. 
    Current Opinion in Biotechnology 17, 394-99.
  10. Fodor, B.D., Kovacs, A.T., Csaki, R., Hunyadi-Gulyas, E., Klement, E., Maroti, G.,Meszaros, L., Medzihradszky, K.F., Rakhely, G. and Kovacs, K.L. (2004) 
    Modular broad-host-range Expression vectors for single-protein and protein complex purification. 
    Appl. Eviroment. Microbiology 70, 712-721.
  11. Witte, C.P., Noël, L.D., Gielbert, J., Parker, J.E. and Romeis, T. (2004) 
    Rapid one-step protein purification from plant material using the eight-amino acid strepII epitope. 
    Plant Molec. Biol. 55, 135-147.
  12. Gross, R., Pisa, R., Sänger, M., Lancaster, C.R.D. and Simon, J. (2004) 
    Characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of Wolinella succinogenes NiFe-hydrogenase. 
    JBC 279, 274-281.
  13. Heinrich, A., Maheswaran, M., Ruppert, U. and Forchhammer, K. (2004) 
    The Synechococcus elongatus pII signal transduction protein controls arginine synthesis by complex formation with N-acetyl-L-glutamate kinase. 
    Molec. Microbiology 52, 1303-1314.
  14. Cotton, M., Stegmueller, K., Eickhoff, J., Hanke, M., Herzberger, K., Herget, T., Choidas, A.,Daub, H. and Godl, K. (2003) 
    Exploiting features of adenovirus replication to support mammalian kinase production. 
    Nucleic Acids Research 31, e128.
  15. Niebisch, A. and Bott, M. (2003) 
    Purification of a cytochrome bc1-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunit of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1. 
    JBC 278, 4339-4346.
  16. Groß, R., Pisa, R., Simon, J. and Kröger, A. (2002) 
    Isolierung der trimeren Hydrogenase aus Wolinella succinogenes durch StrepTactin-Affinitätschromatographie. 
    BIOspektr. 8, 1-2.
  17. Kohlstock, U.-M., Rücknagel, K.P., Reuter, M., Schierhorn, A., Andreesen, J.R. and Söhling,B. (2001) 
    Cys359 of GrdD is the active-site thiol that catalyses the final step of acetyl phosphate formation by glycine reductase from Eubacterium acidaminophilum
    Eur. J. Biochem. 268, 6417-6425.
  18. Müller, S., Dädara, A., Lürsen, K., Wrenger, C., Das Gupta, R., Madhubalai, R. and Walter,R.D. (2000) 
    In the human malaria parasite Plasmodium falciparum, polyamines are synthesized by a bifunctional ornithine decarboxylase, S-adenosylmethionine decarboxylase. 
    JBC 275, 8097-8102.
  19. Zwicker, N., Adelhelm, K., Thiericke, R., Grabley, S. and Hänel, F. (1999) 
    Strep-tag II for one-step affinity purification of active bHLHzip domain of human c-Myc. 
    Biotechniques 27368-27375.
  20. Kleymann, G., Ostermeier, C., Ludwig, B., Skerra, A. and Michel, H. (1995) 
    Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. 
    Bio/Technology 13, 155-160.
  21. Tsiotis, G., Haase, W., Engel, A. and Michel, H. (1995) 
    Isolation and structural characterization of trimeric cyanobacterial photosystem I complex with the help of recombinant antibodyfragments. 
    Eur. J. Biochem. 231, 823-830.

Twin-Strep-tag

  1. Bekker-Jensen S., Rendtlew Danielsen J., Fugger K., Gromova I., Nerstedt A., Bartek J., Lukas J. and Mailand N. (2010)
    HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes
    Nature Cell Biology 12 (1)
  2. Amako Y., Sarkeshik A., Hotta H., Yates J., Siddiqui A. (2009)
    Role of Oxysterol Binding Protein in Hepatitis C Virus infection.
    J Virol. 83(18): 9237–924
  3. Gianni T., Amasio M. and Campadelli-Fiume G. (2009) 
    Herpes simplex virus gD  forms distinct complexes with fusion executors gB  and gH/gL through the  C-terminal profusion domain
    J. Biol. Chem. 284: 17370-1738
  4. Pegoraro G., Kubben N., Wickert U., Göhler H., Hoffmann K. and Misteli T. (2009)
    Ageing-related chromatin defects through loss of the NURD complex.
    Nature Cell Biology 11 : 1261-1267
  5. Oellerich T., Grønborg M., Neumann K., Hsiao H.-H., Urlaub  H. and Wienands J. (2009)
    SLP-65 Phosphorylation Dynamics Reveals A Functional Basis for Signal Integration by Receptor-proximal Adaptor Proteins
    Molecular Cell Proteomics 8 (7):1738-50
  6. Rameix-Welti M.-A., Tomoiu A., Dos Santos Afonso E., van der Werf S., and Naffakh N. (2009)
    Avian Influenza A Virus Polymerase Association with Nucleoprotein, but Not Polymerase Assembly, Is Impaired in Human Cells during the Course of Infection
    J.Virol. 83(3):1320–1331
  7. Bingel-Erlenmeyer, R., Kohler, R., Kramer, G., Sandikci, A., Antolić, S., Maier, T., Schaffitzel, C., Wiedmann, B., Bukau, B. and Ban, N. (2008) 
    A peptide deformylase–ribosome complex reveals mechanism of nascent chain processing. 
    Nature 452, 108-113.
  8. Johansen L.D., Naumanen T., Knudsen A., Westerlund N., Gromova I., Junttila M., Nielsen C., Bøttzauw T., Tolkovsky A., Westermarck J., Coffey E.T., Jäättelä M. and Kallunki T. (2008)
    IKAP localizes to membrane ruffles with filamin A and regulates actin cytoskeleton organization and cell migration. 
    Journal of Cell Science 121, 854-864
  9. Weber M., Wehling M., and Lösel R. (2008)
    Proteins interact with the cytosolic mineralocorticoid receptor depending on the ligand. 
    Am J Physiol Heart Circ Physiol 295, 361–365.
  10. Gloeckner, C.J., Boldt, K., Schumacher, A., Roepman, R. and Ueffing, M. (2007) 
    A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes. 
    Proteomics 7, DOI 10.1002/pmic.200700038
  11. Groth, A.; Corpet, A.; Cook, A.J.L.; Roche, D., Bartek, J.; Lukas, J. and Almouzni, G. (2007) 
    Regulation of replication fork progression through histone supply and demand. 
    Science 318, 1928 – 1931. For supplement see: www.sciencemag.org/cgi/content/full/318/5858/1928/DC1
  12. Morita, E., Sandrin, V., Alam, S., L., Eckert, D., B., Gygi, S., P. and Sundquist, W., I. (2007) 
    Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. 
    Cell Host & Microbe 2, 41-53.
  13. Morita E., Sandrin V., Chung H.-Y., Morham S. G., Gygi S. P., Rodesch C. K. and Sundquist W. I.  (2007) 
    Human ESCRT and ALIX proteins interact with proteins of the midbody and function in Cytokinesis. 
    EMBO Journal 26, 4215–4227.
  14. Junttila, M.R., Saarinen, S., Schmidt, T., Kast, J. and Westermarck, J. (2005) 
    Single-step strep-tag purification for the isolation and identification of protein complexes from mammalian cells. 
    Proteomics 5, 1199-1203.
Enzymes / Metalloenzymes
  1. Bych K., Kerscher S.,.Netz D.J.A , J. Pierik A., Zwicker K., Huynen M. A., Lill R., Brandt U. and Balk J.(2008) 
    The iron–sulphur protein Ind1 is required for effective complex I assembly.
    EMBO Journal 27, 1736–1746.
  2. Sybirna K., Antoine T., Lindberg P., Fourmond V., Rousset M., Méjean V. and Bottin H. (2008)
    Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii. 
    BMC Biotechnology 8:73.
  3. Girbal, L., von Abendroth, G., Winkler, M., Benton, P.M.C., Meynial-Salles, I., Croux, C. and Peters, J.W. (2005) 
    Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and Algal Fe-only hydrogenases with high specific activities. 
    Appl. Enviroment. Microbiology 71, 2777-2781.
  4. Rummel, A., Karnath, T., Henke, T., Bigalke, H. and Binz, T. (2004) 
    Synaptotagmins I and II act as nerve cell receptors for botulinum neurotoxin G. 
    JBC 279, 30865-30870.
  5. Ciubotaru, M., Ptaszek, L.M., Baker, G.A., Baker, S.N., Bright, F.V. and Schatz, D.G. (2003) 
    RAG1-DNA binding in V(D)J recombination. specificity and DNA-induced conformational changes revealed by fluorescence and CD spectroscopy.
    JBC 278, 5584-5596.
  6. Cotton, M., Stegmueller, K., Eickhoff, J., Hanke, M., Herzberger, K., Herget, T., Choidas, A., Daub, H. and Godl, K. (2003) 
    Exploiting features of adenovirus replication to support mammalian kinase production. 
    Nucleic Acids Research 31, e128.
  7. Rummel, A., Bade, S., Alves, J., Bigalke, H. and Binz, T. (2003) 
    Two carbohydrate binding sites in the HCC-domain of tetanus neurotoxin are required for toxicity. 
    J. Mol. Biol. 326, 835-884.
  8. Fontaine, L., Meynial-Salles, I., Girbal, L., Yang, X., Croux, C. and Soucaille, P. (2002) 
    Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. 
    J. Bacteriology 184, 821-830.
  9. Roberts, S.A., Weichsel, A., Grass, G., Thakali, K., Hazzard, J.T., Tollin, G., Rensing, C. and Montfort, W.R. (2002) 
    Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli. 
    PNAS 99, 2766-2771.
  10. Juda, G.A., Bollinger, J.A. and Dooley, D.M. (2001) 
    Construction, overexpression, and purification of Arthrobacter globiformis amine oxidase-strep-tag II fusion protein.
    Prot. Expr. Purific. 22, 455-461.
  11. Kohlstock, U.-M., Rücknagel, K.P., Reuter, M., Schierhorn, A., Andreesen, J.R. and Söhling, B. (2001) 
    Cys359 of GrdD is the active-site thiol that catalyses the final step of acetyl phosphate formation by glycine reductase from Eubacterium acidaminophilum. 
    Eur. J. Biochem. 268, 6417-6425.
  12. Busch, K., Piehler, J. and Fromm, H. (2000) 
    Plant succinic semialdehyde dehydrogenase: dissection of nucleotide binding by surface plasmon resonance and fluorescence spectroscopy.
    Biochemistry 39, 10110-10117.
  13. Hans, M. and Buckel, W. (2000) 
    Purification of recombinant component A of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans using strep-tactin affinitychromatography. 
    Research Update BTi 12.
  14. Hans, M., Buckel, W. and Bill, E. (2000) 
    The iron-sulfur clusters in 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. Biochemical and spectroscopic investigations. 
    Eur. J. Biochem. 267, 7082-7093.
  15. Müller, S., Dädara, A., Lürsen, K., Wrenger, C., Das Gupta, R., Madhubalai, R. and Walter, R.D. (2000) 
    In the human malaria parasite Plasmodium falciparum, polyamines are synthesized by a bifunctional ornithine decarboxylase, S-aAdenosylmethionine decarboxylase. 
    JBC 275, 8097-8102.
  16. Wendt, U.K., Wenderoth, I., Tegeler, A. and von Schaewen, A. (2000) 
    Molecular characterization of a novel glucose-6-phosphate dehydrogenase from potato (Solanum Tuberosum L.). 
    The Plant J. 23, 723-733.
  17. Laber, B., Maurer, W., Scharf, S., Stepusin, K. and Schmidt, F.S. (1999) 
    Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein. 
    FEBS Lett. 449, 45-48.
  18. Maier, T., Drapal, N., Thanbichler, M. and Böck, A. (1998) 
    Strep-tag II affinity purification: an approach to study intermediates of metalloenzyme biosynthesis. 
    Anal. Biochem. 259, 68-73.
  19. Hengsakul, M. and Cass A.E.G. (1997) 
    Alkaline phosphatase-strep-tag fusion protein binding to streptavidin: resonant mirror studies. 
    J. Mol. Biol. 266, 621-632.
Membrane proteins
  1. Müller I.B., Wu F.., Bergmann B., Knöckel J., Walter R.D., Gehring H., Wrenger C. (2009)
    Poisoning Pyridoxal 5-Phosphate-Dependent Enzymes: A New Strategy to Target the Malaria Parasite Plasmodium falciparum. 
    PlosOne 4 (2) e4406.
  2. Ressl S., Terwisscha van Scheltinga A. C., Vonrhein C., Ott  V., Ziegler C. (2009)
    Molecular basis of transport andregulation in the Na1/betaine symporter BetP. 
    Nature 458, 47-53. 
  3. Mobley C. K., Myers J. K., Hadziselimovic A., Ellis C. D., and Sanders C. R. (2007)
    Purification and Initiation of Structural Characterization of Human Peripheral Myelin Protein 22, an Integral Membrane Protein Linked to Peripheral Neuropathies.
    Biochemistry 46 (39), 11185-11195.
  4. Tsai C.-J. and Ziegler C. (2005)
    Structure Determination of Secondary Transport Proteins by Electron Crystallography: Two-Dimensional Crystallization of the Betaine Uptake System BetP. 
    J Mol Microbiol Biotechnol 10, 197–207. 
  5. Gross, R., Pisa, R., Sänger, M., Lancaster, C.R.D. and Simon, J. (2004) 
    Characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of Wolinella succinogenes NiFe-hydrogenase.
    JBC 279, 274-281.
  6. Niebisch, A. and Bott, M. (2003) 
    Purification of a cytochrome bc1- aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum.
    JBC 278, 4339-4346.
  7. Groß, R., Pisa, R., Simon, J. and Kröger, A. (2002) 
    Isolierung der trimeren Hydrogenase aus Wolinella succinogenes durch StrepTactin-Affinitätschromatographie. 
    BIOspektr. 8, 1-2.
  8. Rübenhagen, R., Rönsch, H., Jung, H., Krämer, R. and Morbach, S. (2000) 
    Osmosensor and osmoregulator properties of the betaine carrier BetP from Corynebacterium glutamicum in proteoliposomes. 
    JBC 275, 735-741.
  9. Bungert, S., Krait, B., Schlesinger, R. and Friedrich, T. (1999) 
    One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of strep-tag affinity chromatography. 
    FEBS Letters 460, 207-211.
  10. Goldberg, M., Pribyl, T., Juhnke, S. and Nies, D.H. (1999) 
    Energetics and topology of CzcA, a cation/proton antiporter of the resistance-nodulation-cell division protein family.
    JBC 274, 26065-26070.
  11. Loferer, H., Hammar, M. and Normark, S. (1997) 
    Availabillity of the fibre subunit CsgA and the nucleator protein CsgB during the fibronectin-binding curli is limited by the intracellular concentration of the novel lipoprotein CsgG. 
    Molec. Microbiology 26, 11-23.
  12. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H. (1997) 
    Structure at 2.7 Å resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody Fv fragment. 
    Proc. Natl. Acad. Sci. USA 94, 10547-10553.
  13. Kleymann, G., Ostermeier, C., Ludwig, B., Skerra, A. and Michel, H. (1995) 
    Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. 
    Bio/Technology 13, 155-160.
  14. Tsiotis, G., Haase, W., Engel, A. and Michel, H. (1995) Isolation and structural characterization of trimeric cyanobacterial photosystem I complex with the help of recombinant antibody fragments. 
    Eur. J. Biochem. 231, 823-830.
Antibody fragments
  1. Plewnia G., Schulze K., Hunte C., Tampé R. and Koch J. (2007)
    Modulation of the Antigenic Peptide Transporter TAP byRecombinant Antibodies Binding to the Last FiveResidues of TAP1. 
    J. Mol. Biol. 369, 95–107.
  2. Rau, D., Kramer, K. and Hock, B. (2002) 
    Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecularevolution of variable antibody genes. 
    Anal. Bioanal. Chem. 372, 261-267.
  3. Schlehuber, S. and Skerra, A. (2001) 
    Duocalins: engineered ligand-binding proteins with dual specificity derived from the lipocalin fold. 
    Biol. Chem. 382, 1335-1342.
  4. Beste, G., Schmidt, F.S., Stibora, T. and Skerra, A. (1999) 
    Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. 
    Proc. Natl. Acad. Sci. USA 96, 1898-1903.
  5. Brown, J.C., Brown, B.A. 2nd, Li, Y. and Hardin, C.C. (1998) 
    Construction and characterization of a quadruplex DNA selective single-chain autoantibody from a viable motheaten mouse hybridoma with homology to telomeric DNA binding proteins. 
    Biochemistry 37, 16338-16348.
  6. Koo, K., Foegeding, P.M. and Swaisgood, H.E. (1998) 
    Construction and expression of a bifunctional single-chain antibody against Bacillus cereus spores. 
    Appl. Eviroment. Microbiology 64, 2490-2496.

Applications:

Secretion
  1. Ambrus A., Torocsik B., Adam-Vizi V. (2009)
    Periplasmic cold expression and one-step purification of human dihydrolipoamide dehydrogenase. 
    Protein Expression and Purification 63, 50-57.
  2. Smits S. H. J., Höing M., Lecher J., Jebbar M., Schmitt L., and Bremer E. (2008) 
    The Compatible-Solute-Binding Protein OpuAC from Bacillus subtilis: Ligand Binding, Site-Directed Mutagenesis, and Crystallographic Studies. 
    Journal of Bacteriology 190 (16), 5663–5671.
  3. Meng G., Surana N. K., St Geme III J. W. and Waksman G. (2006) 
    Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter. 
    EMBO Journal 25, 2297–2304.
  4. Humtsoe, J.O., Kim, J.K., Xu, Y., Keene, D.R., Höök, M., Lukomski, S. and Wary, K.K. (2005) 
    A streptococcal collagen-like protein interacts with the integrin and induces intracellular signalling. 
    JBC 280, 13848-13857.
  5. Cass, B., Pham, P.L., Kamen, A. and Durocher, Y. (2004) 
    Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. 
    Prot. Expr. Purific. 40, 77-85.
  6. Xu, Y., Keene, D.R., Bujnicki, J., Höök, M. and Lukomski, S. (2002) 
    Streptococcal Scl1 and Scl2 proteins form collagen-like triple helices. 
    JBC 277, 27312-27318.
  7. Klatt, A.R., Nitsche, D.P., Kobbe, B., Macht, M., Paulsson, M. and Wagener R. (2001) 
    Molecular structure, processing, and tissue distribution of matrilin-4. 
    JBC 276, 17267-17275.
  8. Streuli, C.H. and Grant, M.E. (2000) 
    Extracellular matrix protocols. 
    Methods Molec. Biol. 47-57.
  9. Tudyka, T. and Skerra, A. (1997) 
    Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli. 
    Protein Science 6, 2180-2187.
Assays/HTS
  1. Ernst, W.J., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R.M. (2000) 
    Expanding baculovirus surface display modification of the native coat protein Gp64 of autographa californica NPV. 
    Eur. J. Biochem. 267, 4033-4039.
  2. Panke, O., Gumbiowski, K., Junge, W. and Engelbrecht, S. (2000) 
    F-ATPase: specific observation of the rotating c subunit oligomer of EFoEF1. 
    FEBS Letters 472, 34-38.
  3. Skerra, A. and Schmidt, T.G.M. (1993) 
    Use of the strep-tag and streptavidin for detection and purification of recombinant proteins. 
    Methods in Enzymology 326, 271-304.
  4. Sebastian, P., Wallwitz, J. and Schmidt, S. (2003) 
    Semi automated production of a set of different recombinant GST-streptag fusion proteins.
    J. Chromatogr. B 786, 343-355
Pulldowns

Strep-tag

Danielsen J. M. R., Sylvestersen K. B., Bekker-Jensen S., Szklarczyk D., Poulsen J. W., Horn H., Jensen L.J., Mailand N. and Nielsen M. L. (2011)
Mass Spectrometric Analysis of Lysine Ubiquitylation Reveals Promiscuity at Site Level*
Molecular & Cellular Proteomics 10.3; doi10.1074/mcp.M110.003590

 

Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid K. A. S., Grill E., Romeis T. and Hedrich  R. (2010) 
Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities
PNAS 107 (17):8023–8028

 

Twin-Strep-tag

HORII M., SHIBATA H., KOBAYASHI R., KATOH K., YORIKAWA C., YASUDA J. and MAKI M. (2006) 
CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway
Biochem. J. 400: 23–32; doi:10.1042/BJ20060897

 

Pegoraro G., Kubben N., Wickert U., Göhler H., Hoffmann K. and Misteli T. (2009)
Ageing-related chromatin defects through loss of the NURD complex.
Nature Cell Biology 11 : 1261-1267

 

Gianni T., Amasio M. and Campadelli-Fiume G. (2009) 
Herpes simplex virus gD  forms distinct complexes with fusion executors gB  and gH/gL through the  C-terminal profusion domain
J. Biol. Chem. 284: 17370-1738

Protein-Protein interactions (Twin-Strep-tag®)
  1. Varjosalo M, Sacco R, Stukalov A, van Drogen A, Planyavsky M, Hauri S, Aebersold R, Bennett KL, Colinge J, Gstaiger M, Superti-Furga G. (2013)
    Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS
    Nat Methods., 10(4), 307-14. doi: 10.1038/nmeth.2400
  2. Jasencakova Z, Scharf AND, Ask K, Corpet A, Imhof A, Almouzni G, Groth A, (2010)
    Replication stress interferes with histone recycling and predeposition marking of new histones.
    Molecular Cell37: 736–743
  3. Bekker-Jensen S., Rendtlew Danielsen J., Fugger K., Gromova I., Nerstedt A., Bartek J., Lukas J. and Mailand N. (2010)
    HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes
    Nature Cell Biology12 (1)
  4. Amako Y., Sarkeshik A., Hotta H., Yates J., Siddiqui A. (2009)
    Role of Oxysterol Binding Protein in Hepatitis C Virus infection.

    J Virol. 83(18): 9237–924
  5. Gianni T., Amasio M. and Campadelli-Fiume G. (2009) 
    Herpes simplex virus gD  forms distinct complexes with fusion executors gB  and gH/gL through the C-terminal profusion domain
    J. Biol. Chem.284: 17370-1738
  6. Neumann K, Oellerich T, Urlaub H, Wienands J. (2009)
    The Blymphoid Grb2 interaction code.
    Immunol Rev 232: 135-149
  7. Pegoraro G., Kubben N., Wickert U., Göhler H., Hoffmann K. and Misteli T. (2009)
    Ageing-related chromatin defects through loss of the NURD complex.
    Nature Cell Biology 11 : 1261-1267
  8. Oellerich T., Grønborg M., Neumann K., Hsiao H.-H., Urlaub  H. and Wienands J. (2009)
    SLP-65 Phosphorylation Dynamics Reveals A Functional Basis for Signal Integration by Receptor-proximal Adaptor Proteins
    Molecular Cell Proteomics8 (7):1738-50
  9. Rameix-Welti M.-A., Tomoiu A., Dos Santos Afonso E., van der Werf S., and Naffakh N. (2009)
    Avian Influenza A Virus Polymerase Association with Nucleoprotein, but Not Polymerase Assembly, Is Impaired in Human Cells during the Course of Infection
    J.Virol. 83(3):1320–1331
  10. Bingel-Erlenmeyer, R., Kohler, R., Kramer, G., Sandikci, A., Antolić, S., Maier, T., Schaffitzel, C., Wiedmann, B., Bukau, B. and Ban, N. (2008) 
    A peptide deformylase–ribosome complex reveals mechanism of nascent chain processing. 
    Nature452, 108-113.
  11. Johansen L.D., Naumanen T., Knudsen A., Westerlund N., Gromova I., Junttila M., Nielsen C., Bøttzauw T., Tolkovsky A., Westermarck J., Coffey E.T., Jäättelä M. and KallunkiT. (2008)
    IKAP localizes to membrane ruffles with filamin A and regulates actin cytoskeleton organization and cell migration. 
    Journal of Cell Science121, 854-864
  12. Weber M., Wehling M., and Lösel R. (2008)
    Proteins interact with the cytosolic mineralocorticoid receptor depending on the ligand. 
    Am J Physiol Heart Circ Physiol295, 361–365.
  13. Gloeckner, C.J., Boldt, K., Schumacher, A., Roepman, R. and Ueffing, M. (2007) 
    A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes. 
    Proteomics7, DOI 10.1002/pmic.200700038
  14. Groth, A.; Corpet, A.; Cook, A.J.L.; Roche, D., Bartek, J.; Lukas, J. and Almouzni, G. (2007) 
    Regulation of replication fork progression through histone supply and demand. 
    Science318, 1928 – 1931. For supplement
  15. Morita, E., Sandrin, V., Alam, S., L., Eckert, D., B., Gygi, S., P. and Sundquist, W., I. (2007) 
    Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. 
    Cell Host & Microbe2, 41-53.
  16. Morita E., Sandrin V., Chung H.-Y., Morham S. G., Gygi S. P., Rodesch C. K. and Sundquist W. I.  (2007) 
    Human ESCRT and ALIX proteins interact with proteins of the midbody and function in Cytokinesis. 
    EMBO Journal26, 4215–4227.
  17. Junttila, M.R., Saarinen, S., Schmidt, T., Kast, J. and Westermarck, J. (2005) 
    Single-step strep-tag purification for the isolation and identification of protein complexes from mammalian cells. 
    Proteomics5, 1199-1203.
Protein:DNA(RNA) interactions
  1. Abe H., Miyahara A., Oshima T., Tashiro K., Ogura Y., Kuhara S. Ogasawara N., Hayashi T. and Tobe T. (2008)
    Global Regulation by Horizontally Transferred Regulators Establishes the Pathogenicity of Escherichia coli
    DNA Research 15, 25–38.
  2. Wenta N., Strauss H., Meyer S., and Vinkemeier U. (2008)
    Tyrosine phosphorylation regulates the partitioning of STAT1 between different dimer conformations. 
    PNAS 105 (27), 9238–9243.
Crystallization

Strep-tag still attached

  1. Lukat P., Hoffmann M. and Einsle O. (2008)
    Crystal packing of the c6-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II. 
    Acta Cryst. D64, 919–926.
  2. Marienhagen et al. (2008)
    Insights into the structural basis of substrate recognition by histidinol-phosphate aminotransferase from Corynebacterium glutamicum. 
    Acta Crystallogr D Biol Crystallogr. 64, 675-85.
  3. Schönfeld D. L., Ravelli R. B.G., Mueller U. and Skerra A. (2008)
    The 1.8 Å crystal structure of alpha1-acid glycoprotein (orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. 
    J. Mol. Biol. 384, 393–405.
  4. Jin et al. (2006) 
    Botulinum neurotoxin B recognizes its protein receptor with high affinity and specificity. 
    Nature 444, 1092-1095.
  5. Stummeyer et al. (2005) 
    Crystal structure of the polysialic acid–degrading endosialidase of bacteriophage K1F. 
    Nature Structural & Molecular Biology 12, 90-96.
  6. Korndörfer, I.P., Dommel, M.K. and Skerra, A. (2004) 
    Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture. 
    Nature. Struct. Mol. Biol. 11, 1015-1020.
  7. Lange C., Hunte C. (2002) 
    Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c. 
    Proc. Natl. Acad. Sci. USA  99, 2800-2805.
  8. Hunte C., Köpke J., Lange C., Roßmanith T., Michel H. (2000) 
    Structure at 2.3 Å resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment.
    Structure 8, 669-684.
  9. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H. (1997) 
    Structure at 2.7 Å resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody Fv fragment.
    Proc. Natl. Acad. Sci. USA 94, 10547-10553.
  10. Redondo P., Prieto J., Munoz I. G., Alibé A., Stricher F., Serrano L., Cabaniols J.-P., Daboussi F., Arnould S., Perez C., Duchateau P., Paques F., Blanco F. J.,.Montoya G
    Molecular basis of xeroderma pigmentosum group CDNA recognition by engineered meganucleases. (2008)
    Nature 456, 107-111.
  11. Ressl S., Terwisscha van Scheltinga A. C., Vonrhein C., Ott  V., Ziegler C. (2009)
    Molecular basis of transport andregulation in the Na1/betaine symporter BetP. 
    Nature 458, 47-53.
  12. Ilk, N., Völlenkle, C., Egelseer, E.M., Breitwieser, A., Sleytr, U.B. and Sara, M. (2002) 
    Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen. 
    Appl. Enviroment. Microbiology 68, 3251-3260.
  13. Roberts et al. (2002) 
    Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli
    PNAS 99, 2766-2771. 
  14. Roberts, S.A., Weichsel, A., Grass, G., Thakali, K., Hazzard, J.T., Tollin, G., Remsing, C. and Montfort, W.R. (2002) 
    Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli. 
    PNAS 99, 2766-2771.
  15. Locher et al. (2001) 
    Crystal structure of the Acidaminococcus fermentans 2- hydroxyglutaryl-CoA dehydratase component A. 
    J. Mol. Biol. 307, 297-308.
  16. Ostermeier, C., Essen, L.O. and Michel, H. (1995) 
    Crystals of an antibody Fv fragment against an integral membrane protein diffracting to 1.28 Å resolution. 
    Proteins 21, 74-77.
  17. Ostermeier, C., Iwata, S., Ludwig, B. and Michel, H. (1995) 
    Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. 
    Nature. Struct. Biol. 2, 842-846.

attachement of tag not stated

  1. Redondo P., Prieto J., Munoz I. G., Alibé A., Stricher F., Serrano L., Cabaniols J.-P., Daboussi F., Arnould S., Perez C., Duchateau P., Paques F., Blanco F. J.,.Montoya G (2008)
    Molecular basis of xeroderma pigmentosum group CDNA recognition by engineered meganucleases.
    Nature 456, 107-111.
  2. Ressl S., Terwisscha van Scheltinga A. C., Vonrhein C., Ott  V., Ziegler C. (2009)
    Molecular basis of transport andregulation in the Na1/betaine symporter BetP. 
    Nature 458, 47-53.
  3. Ilk, N., Völlenkle, C., Egelseer, E.M., Breitwieser, A., Sleytr, U.B. and Sara, M. (2002) 
    Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen. 
    Appl. Enviroment. Microbiology 68, 3251-3260.
  4. Roberts et al. (2002) 
    Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli
    PNAS 99, 2766-2771. 
  5. Roberts, S.A., Weichsel, A., Grass, G., Thakali, K., Hazzard, J.T., Tollin, G., Remsing, C. and Montfort, W.R. (2002) 
    Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli. 
    PNAS 99, 2766-2771.
  6. Locher et al. (2001) 
    Crystal structure of the Acidaminococcus fermentans 2- hydroxyglutaryl-CoA dehydratase component A. 
    J. Mol. Biol. 307, 297-308.
  7. Ostermeier, C., Essen, L.O. and Michel, H. (1995) 
    Crystals of an antibody Fv fragment against an integral membrane protein diffracting to 1.28 Å resolution. 
    Proteins 21, 74-77.
  8. Ostermeier, C., Iwata, S., Ludwig, B. and Michel, H. (1995) 
    Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. 
    Nature. Struct. Biol. 2, 842-846.
Detection
  1. Müller I. B., Knöckel J., Groves M. R., Jordanova R., Ealick S. E., Walter R.D., Wrenger C. (2008)
    The Assembly of the Plasmodial PLP Synthase Complex Follows a Defined Course. 
    PlosOne 3 (3), e1815.
  2. Ichioka F., Kobayashi R., Katoh K., Shibata H. and Maki M. (2008)
    Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4). 
    FEBS Journal 275 (4), 682-692.
  3. Kurdyukov S., Faust A., Nawrath C., Bär S., Voisin D., Efremova N., Franke R., Schreiber L., Saedler H., Métraux J.-P., and Yephremova A. (2006)
    The Epidermis-Specific Extracellular BODYGUARD Controls Cuticle Development and Morphogenesis in Arabidopsis. 
    The Plant Cell 18, 321–339.
  4. Rummel, A., Mahrhold, S., Bigalke H. and Binz, T. (2003) 
    The HCC-domain of botulinum neurotoxins A and B exhibits a singular ganglioside binding site displaying serotype specific carbohydrate interaction. 
    Molec. Microbiology 51, 631-643
  5. Ribrioux, S., Kleymann, G., Haase, W., Heitmann, K., Ostermeier, C. and Michel, H. (1996) 
    Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunochemistry. 
    The Journal of Histochemistry and Cytochemistry 44, 207-213.
  6. Kleymann, G., Iwata, S., Wiesmüller, H.H., Ludwig, B. and Michel, H. (1995) 
    Immunoelectronic microscopy and epitope mapping with monoclonal antibodies suggests the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases. 
    Eur. J. Biochem. 230, 359-363.
  7. Kleymann, G., Ostermeier, C., Heitmann, K., Haase, W. and Michel H. (1995) 
    Use of antibody fragments (Fv) in immunochemistry. 
    The Journal of Histochemistry and Cytochemistry 43, 607-614.
  8. Tsiotis, G., Haase, W., Engel, A. and Michel, H. (1995) 
    Isolation and structural characterization of trimeric cyanobacterial photosystem I complex with the help of recombinant antibody fragments. 
    Eur. J. Biochem. 231, 823-830.