Root > Products > Strep-Tactin® & Strep-tag® > Strep-Tactin Resins > Superflow (high capacity) > Strep-Tactin Superflow Resins for Protein Purification Using Gravity Flow, Low Pressure or FPLC

Strep-Tactin® Superflow®

Strep-Tactin® Superflow® & Strep-Tactin® Superflow® high capacity

Both Strep-Tactin® Superflow® resins:

  • combine outstanding flow characteristics with superior mechanical stability
  • are suitable for increased flow rates and useful for purification of large protein complexes
  • yield much higher protein purity (> 95 %) than 6xHistidine-tag purification on Ni-NTA Superflow®

Both resins are available as:

  • 50 % suspension (slurry)
  • pre-packed Strep-Tactin® gravity flow columns
  • pre-packed cartridges for work with automated workstations like Äkta

Difference between Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity:

Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the perfect solution for Strep-tag® fusion proteins at high expression levels.

 

The Strep-Tactin® Superflow® high capacity resin

  • provides 2 to 3 times higher binding capacity than standard Strep-Tactin® Superflow® (150 – 500 nmol or up to 15 mg recombinant Strep-tag®II fusion protein per ml sedimented resin)
  • enables a cost effective purification of higher protein amounts at optimal purity
  • isolation of comparable protein yields with His-tag/Ni-NTA purification

 

 

Fig1: Comparison of Strep-Tactin® Superflow® with Ni-NTA Superflow® in respect to binding capacity and protein purity.