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Strep-Tactin® purification resins

Several Strep-Tactin® resin versions ( Sepharose®, Superflow®, Superflow® high capacity and MacroPrep®) are available which differ in their properties and suitability for applications:

Sepharose®
  • The resin to start with (if you have no requirements)!
  • Agarose support with good flow properties
  • For gravity flow chromatography only (not pressure stable)

Superflow®

  • Our all-rounder for large proteins and protein:protein interac­tion analysis
  • Agarose support with low unspecific binding properties
  • For increased flow rates
  • Low pressure stable (FPLC/HPLC/Äkta applications)

Superflow® high capacity (HC)

  • The capacity of Superflow® is not sufficient or the binding affinity to (Twin-)Strep-tag® fusion
  • proteins needs to be slightly improved!
  • 3-5 fold higher binding capacity as Superflow®
MacroPrep®
  • The alternative if Sepharose® and Superflow® lead to non-spe­cific binding of protein
  • e.g. Chlorophyll from plant extracts
  • Synthetic support with low non-specific binding properties
  • Highly pressure stable (suitablel for FPLC/HPLC /Äkta applications)

For batch purification use our MagStrep "type3" XT beads- magnetic beads with high specificity for low unspecifc background.

Strep-Tactin® Resin specifications
  Strep-Tactin® Sepharose® Strep-Tactin® Superflow®

Strep-Tactin® Superflow® High capacity (HC)

Strep-Tactin® MacroPrep®
Gravity flow column
Yes Yes Yes Yes
batch purification
No
No No
No
FPLC No
Yes Yes Yes
HPLC
No
Yes Yes Yes
Binding capacity
50 - 100 nmol/ml 50 - 100 nmol/ml 150 - 500 nmol/ml 50 - 100 nmol/ml
Support Sepharose 4 FF
Superflow 6
Superflow 6
MacroPrep 50
Bead structure
agarose
6% agarose, crosslinked
6% agarose, crosslinked polymethacrylate
Bead size
45 - 165 µm 60 - 160 µm spherical 60 - 160 µm spherical 50 µm
Exclusion limit
3 x 107 6 x 106 6 x 106 1 x 106
Recommended linear flow rate* up to 30 cm/h up to 300 cm/h up to 300 cm/h up to 300 cm/h
pH stability 4-9
2-13
2-13
2-12
Max pressure Gravity flow 9,6 bar 9,6 bar 70 bar (cartridge cover 20 bar only)
Form buffered 50% suspension buffered 50% suspension buffered 50% suspension buffered 50% suspension
Storage 4 °C, do not freeze 4 °C, do not freeze 4 °C, do not freeze 4 °C, do not freeze
Shipment RT
RT
RT
RT
Buffers and reagents for the Strep-tag® /Strep-Tactin® purification system
Reagents compatible with the Strep-tag®/Strep-Tactin® interaction
Reduction Agents Concentration
DTT 50 mM
β-mercaptoethanol 50 mM
Non-Ionic Detergents  
C8E4 Octyltetraoxyethylene max. 0.88 %
C10E5; Decylpentaoxyethylene 0.12 %
C10E6 0.03 %
C12E8 0.005 %
C12E9; Dodecyl nonaoxyethylene (Thesit) 0.023 %
DM; Decyl-β-D-maltoside 0.35 %
DDM or (LM); N-dodecyl-β-D-maltoside 2 %
Digitonin 0,5 %
NG; N-nonyl-β-D-glucopyranoside 0.2 %
NP40; Nonidet P40 2 %
OG; N-octyl-β-D-glucopyranoside 2.34 %
TX; Triton X-100 2 %
Tween 20 2 %
Ionic Detergents  
N-lauryl-sarcosine 2 %
8-HESO;N-octyl-2-hydroxy-ethylsulfoxide 1,32 %
SDS; Sodium-N-dodecyl sulfate 0.1 %
Zwitter-Ionic Detergents  
CHAPS 0.5 %
DDAO; N-decyl-N,N-dimethylamine-N-oxide 0.034 %
Fos-Choline-12 0,1 %
LDAO; N-dodecyl-N,N-dimethylamine-N-oxide 0.13 %
Others  
Ammonium sulfate (NH4)2SO4 2 M
CaCl2 max. 1 M
CHS; Cholesterol hemisuccinate 0,1 %
EDTA 50 mM
Ethanol 10 %
Guanidine max. 1 M
Glycerol max. 25 %
Imidazole max. 250 mM
MgCl2 1 M
NaCl 5 M
Urea max. 1 M