New applications of the 3rd generation Strep-tag® system

Strep-Tactin®XT:Twin-Strep-tag® is the most versatile affinity purification system and can be used for all kind of applications:

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Batch purification

For batch purification IBA provides Strep-Tactin®XT coated magnetic beads: MagStrep "type3" XT beads. MagStrep "type3" XT beads allow the batch purification of highly pure proteins in Strep-tag® quality and the possibility of different elution procedures:

  • Native conditions using biotin
  • Denaturing conditions using sample buffer and boiling for elution
For more information about our MagStrep "type 3" beads click here.
 
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For quick and dirty test purification in batch format you can also use Strep-Tactin®XT Superflow resin.

Purification under denaturing conditions

The 3rd generation Strep-tag® system can now be used for purification under denaturing conditions using up to 6 M urea.

Check the application note for a comparison of Strep-tag® system III to His-tag:NiNTA purification under denaturing conditions.

Download experimental data:

Strep-Tactin®XT under denaturing conditions

Download protocol:

Purification with Strep-Tactin®XT under denaturing conditions

High-throughput screening

Recommended products for high-throughput applications:

Purification of integral membrane proteins with Strep-Tactin®XT

The novel high affinity resin Strep-Tactin®XT was sucessfully tested for purification of integral membrane proteins:

Application of Strep-Tactin®XT for affinity purification of Twin-Strep-tagged CB2, a G protein-coupled cannabinoid receptor (Yeliseev et al., 2016)