Tet expression system
Features and benefits of the pASK-IBA vectors:
- High-level expression in E. coli
- Tightly regulated expression due to the tetracycline promoter
- Enhanced stability of cytotoxic genes
- Inexpensive induction with anhydrotetracycline
The pASK-IBA vectors are available with Strep-tag®II or 6xHis-tag, the OmpA secretion signal, special protease cleavage sites or Chloramphenicol resistance.
Principle and properties
pASK-IBA vectors work with the tightly regulated tetracycline (tet) promoter. Expression of the foreign gene is stringently repressed until induction with a low concentration of the chemical anhydrotetracycline. In contrast to the lac promoter, the tetA promoter/operator is tightly controlled and not functionally coupled to any cellular regulation mechanisms or genetic background. Unlike with the T7 promoter, special E. coli strains or extra plasmids are not required. The vectors do not mediate resistance against tetracycline.