pEXPR-IBA vectors with Strep-tag® for mammalian expression
pEXPR-IBA vectors from the Classic Cloning series for expression of Strep-tagged proteins in mammalian cells are available with different protease cleavage sites.
The vectors provide the same cloning strategy as for the pASK-IBA vectors and are, thus, compatible with the corresponding bacterial pASK-IBA plasmids.
As a result, a PCR fragment can be cloned into pASK-IBA and its pEXPR-IBA equivalent in parallel (e.g. pASK-IBA7plus and pEXPR-IBA7).
The human cytomegalovirus (CMV) immediate early promoter provides strong expression in a wide range of mammalian cells. To prolong expression in transfected cells, the vector will replicate in cell lines that are latently infected with SV40 large T-antigen (e.g. COS7). In addition, the Neomycin resistance gene allows direct selection of stable cell lines.
|Strep-tag® II||Purification of recombinant protein using Strep-Tactin® matrices|
|CMV immediate-early promoter/enhancer||High-level expression in a wide range of mammalian cells|
|Multiple cloning site||Insertion of gene of interest and fusion to Strep-tag®. Compatible with pASK-IBA vectors|
|Neomycin resistance gene||Selection of stable transfectants in mammalian cells|
|Ampicillin resistance gene||Selection in E. coli|
|pUC origin||High-copy number replication in E. coli|
Factor Xa, thrombin (thb),
enterokinase (ent) and
TEV cleavage sites
|Removal of the Strep-tag® if required (generally not necessary)|