6-5000-001

Strep-Tactin® PE or APC

Fluorophore-conjugated Strep-Tactin® for cell staining

€293.00*

Strep-Tactin® Conjugate
Strep-Tactin® conjugated to either R-Phycoerythrin (PE) or Allophycocyanin (APC) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®) based on our Strep-tag® technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells’ full biological activity.

Specifications

Form: Suspension in DPBS
Possible Application: Fluorescent cell staining & sorting
Recommended Usage: 1 µl per 2-3 x 10^6 total cells

Shipping information

Storage: 2-8 °C; Do not freeze!
Stability: 12 months after shipping
Shipping: Blue ice

Accessories

Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
10x Buffer CI
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA
MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
CD3 Fab-Strep, human
Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD3

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