2-4151-000

Strep-Tactin®XT Spin Column Kit

All reagents for rapid purification of Twin-Strep-tag® and Strep-tag®II fusion proteins

€160.00*

Content
Strep-Tactin®XT Spin Column Kit contains all essential reagents required for purification of Strep-tag®II and Twin-Strep-tag® fusion proteins from small samples. It provides Buffer W, Buffer BXT, 25 spin columns and receiver tubes. Spin columns are filled with dried polymethacrylate, a synthetic material coupled with the streptavidin variant Strep-Tactin®XT and are compatible with conventional microcentrifuges, allowing a rapid, parallel processing of multiple samples.
With one column up to 400 µg of mCherry-Strep-tag®II (28 kDa) can be purified under physiological conditions in less than 10 minutes. Prior to application, the spin columns need to be re-hydrated and activated with Buffer W. In contrast to agarose resins, plant cell components such as chlorophyll are not unspecifically bound by the resin, making the Strep-Tactin®XT Spin Column Kit a favorable alternative.
Please note that the spin columns are not reusable.

Specifications

Kit Content: 25 spin columns, 25 receiver tubes, once 1x Buffer W and 1x Buffer BXT
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag® and Strep-tag®II
Support: Polymethacrylate
Twin-Strep-tag® Binding Capacity: 400 µg/spin column

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Related Products

MagStrep "type3" XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT Spin Columns
For rapid purification of Twin-Strep-tag and Strep-tag®II fusion proteins from small samples