Antigen-specific Cell Staining - Products

The MHC I Streptamers®, adducts of Strep-tagged MHC I molecules (MHC I-Streps) and Strep-Tactin®, are directed to antigen-specific CD8 T cell receptors.

Cell staining procedure


1) Peptide (antigen)-loaded MHC I-Streps (Strep-tagged MHC I molecules) are multimerized with Strep-Tactin® attached to a fluorochrome to generate multimers with high binding avidity. These multimers are determined for staining of T cells which are specific for the respective peptide-loaded MHC molecule.

 

2) After FACS analysis or FACS sorting of the target cells, the staining reagents can be completely removed with biotin under physiological conditions at low temperatures (4°C). The monomeric low affinity MHC I-Strep molecules are then dissociated completely from the cell surface by two short washing steps. Target cells are left phenotypically and functionally indistinguishable from untreated cells.

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
D-Biotin stock solution for Streptamers 1 ml 6-0219-001 USD 40.00

Biotin stock solution for dissociation of Streptamer® reagents from cells.

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Strep-Tactin PE for MHC I Streptamers 50 tests a 1 to 2 x10^6 cells 6-5000-001 USD 366.00
6-5000-001

For staining/FACS isolation of antigen-specific cytotoxic T cells (CD8). To complete the staining reaction a MHC I-Strep (of choice) is required in addition to the fluorescent Strep-Tactin. ...

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Strep-Tactin APC for MHC I Streptamers 50 tests a 1 to 2 x10^6 cells 6-5010-001 USD 366.00

For staining/FACS isolation of antigen-specific cytotoxic T cells (CD8). To complete the staining reaction a MHC I-Strep (of choice) is required in addition to the fluorescent Strep-Tactin. ...

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Nylon filter tubes for Streptamer cell isolation 10 filter tubes 6-5601-010 USD 72.00  
6-5601-010

Nylon filter tubes for Streptamer® cell selection.

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Buffer IS for Streptamers 50 ml, 10 x concentrated 6-5602-050 USD 60.00  

Buffer IS, 10x concentrated; Streptamer washing buffer

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Streptamer® Solution Set Standard for washing und dissociation Buffer IS, Biotin 6-5603-005 USD 87.00  

Streptamer® Solution Set Standard for washing and dissociation of Streptamer reagents from cells. ...

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Streptamer® Solution Set XL for washing und dissociation Buffer IS, Biotin 6-5603-055 USD 445.00  

Streptamer® Solution Set Standard for washing and dissociation of Streptamer reagents from cells. ...

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MHC I-Strep HLA-A*0201; CMV pp65; NLVPMVATV Fluorescent staining: 50 tests a 1 to 2 x10^6 cells; Magnetic isolation: 5 preps a 2x10^7 cells 6-7001-001 USD 560.00

For fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. ...

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MHC I-Strep HLA-A*0201; EBV BMLF-1; GLCTLVAML Fluorescent staining: 50 tests a 1 to 2 x10^6 cells Magnetic isolation: 5 preps a 2x10^7 cells 6-7002-001 USD 560.00

For fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. ...

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MHC I-Strep HLA-A*0201; Influenza M; GILGFVFTL Fluorescent staining: 50 tests a 1 to 2 x10^6 cells Magnetic isolation: 5 preps a 2x10^7 cells 6-7003-001 USD 560.00

For fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. ...

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MHC I-Strep HLA-A*0201; HIV gag; SLYNTVATL Fluorescent staining: 50 tests a 1 to 2 x10^6 cells Magnetic isolation: 5 preps a 2x10^7 cells 6-7004-001    
 

For fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. ...

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MHC I-Strep HLA-A*0201; HIV pol; ILKEPVHGV Fluorescent staining: 50 tests a 1 to 2 x10^6 cells Magnetic isolation: 5 preps a 2x10^7 cells 6-7005-001 USD 560.00

For fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. ...

Further details, manuals, data sheets, related products and more