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Antigen-specific T cell staining with reversible MHC I Streptamers®

Reversible MHC I Streptamers® for fluorescent staining and FACS sorting of antigen-specific CD8+ T cells from PBMCs with Strep-Tactin® PE or APC.

What you need:

Fluorescent labelling with MHC I Streptamers®
FACS cell sorting with MHC I Streptamers®
Removal of MHC I Streptamers® after cell sorting
MHC I Streptamer® cell staining results in functional cells

Fluorescent MHC I Streptamer®

Components of the fluorescent MHC I Streptamer® complex
Components of the fluorescent MHC I Streptamer® complex
Summary MHC I Streptamer® cell staining

Fluorescent MHC I Streptamer®

Reagents:
  • Antigen-specific MHC I-Streps; choose among a large range of different alleles and antigens
  • Strep-Tactin® PE or APC
  • Buffer IS, 10x solution
  • Biotin stock solution