Antigen-specific T cell staining with reversible MHC I Streptamers®

Reversible MHC I Streptamers® for fluorescent staining and FACS sorting of antigen-specific CD8+ T cells from PBMCs with Strep-Tactin® PE or APC.

What you need:

Workflow of the fluorescent staining with MHC I Streptamers®

Fluorescent MHC I Streptamer®

Components of the fluorescent MHC I Streptamer® complex
Components of the fluorescent MHC I Streptamer® complex

Fluorescent MHC I Streptamer®

Reagents:
  • Antigen-specific MHC I-Streps; choose among a large range of different alleles and antigens
  • Strep-Tactin® PE or APC
  • Buffer IS, 10x solution
  • Biotin stock solution