Application data Fab Streptamers® for T cell isolation

Sequential positive selection of human CD3+ CD4+ T cells from whole blood with the reversible Fab Streptamers®

The reversibility of Streptamer® reagents allow sequential isolation of specific cell populations
The reversibility of Streptamer® reagents allow sequential isolation of specific cell populations.

PBMCs were isolated with a Ficoll gradient from whole blood. In the first selection step, the CD3 Fab Streptamer® Isolation Kit MB, human  (6-8000-201) was used: PBMCs were incubated with CD3 Fab-Streps conjugated to magnetic microbeads in order to pre-select CD3+ cells. The resulting positive fraction was then treated with D-biotin and washed to remove all labeling reagents. In the second selection step, the CD4 Fab Streptamer® Isolation Kit MB, human  (6-8000-206) was used: CD4+ cells were enriched from the pre-selected CD3+ T cell pool with CD4 Fab-Streps conjugated to magnetic microbeads. Live cells of each selection step are shown. Dead cells were excluded from the analysis with DAPI.

Serial positive selections of lymphocytes and rare subpopulations with the reversible Fab Streptamers®

Application Cell Marker, human Application Note
Positive isolation of human
CD3 T cells
CD3 Application Note CD3, pdf
Positive isolation of human
CD8 Cytotoxic T cells
CD8 Application Note CD8, pdf
Tandem purification of human
low frequency antigen-specific
CD8+ T cell subsets
CD8 and MHC I peptides Application Note tandem, pdf
Multiparameter cell selection
of human Central Memory T cells
CD8, CD62L, CD45RA- Application Note Tcm, pdf
Discriminate between human
Naive and Memory (CD45RA-) T cells
CD8, CD62L, CD45RA- Application Note Tcm, pdf
Triple positive isolation of human
Regulatory T cells
CD4, CD25, CD45RA Application Note Treg, pdf
Helper T cell CD4