Root > Products > His-STREPPER > His-STREPPER > Application example of His-STREPPER

Application example

His-STREPPER improves purification of 6xHis-tag fusion proteins

Purification of a GFP-6xHis-tag fusion protein from crude bacterial lysate using different protocols.
His-STREPPER/Strep-Tactin® provides by far purer proteins than 6xHis-tag/Ni-NTA under physiological conditions (PBS pH 8.0; upper panel) and even better purity than 6xHis-tag/Ni-NTA under optimized buffer conditions (NaCl/imidazole; lower panel).

Protein purifi cation analysis was performed with an Agilent Bioanalyzer 2100 system instead of SDS-PAGE.

Click to enlarge the figure