FABian® - Application data
Cell Selection Process using FABian®
First, FABian® is equipped with the Fab-TACS® Auto column filled with a Strep-Tactin® coated cell-grade agarose matrix (see fig. below) and reagent tubes containing the sample and the required separation reagents. FABian® starts the cell separation process by loading low-affinity Strep-tagged Fab fragments onto the matrix, e.g. a CD3 Fab-Strep for the selection of T cells. Subsequently, FABian® passes whole blood, mouse spleen cells or other single-cell suspensions through the column. Target cells adhere to the affinity matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are washed away efficiently.
Advantages at a glance:
Isolation of PBMC-like and T cells from human whole blood
For more information please watch our video.
Please download your application note of interest:
|FABian® – Lymphocyte isolation|
The first row of the image shows a 0.12 % share of
PBMC-like cells (CD81-positive) in the whole blood sample before the separation
The second row presents the results of the positive fraction after separation, indicating an excellent purity of 95 % and a high yield of the PBMC-like cells (targeted CD81 positive).
FABian® in use
See how FABian® is used in the department of Experimental Transfusion Medicine at TU Dresden:
FABian® Technical Support
Contact us by phone: +49 (0)341 - 99 38 98 29
Don't hesitate to contact us for further questions or make an appointment to learn more about FABian®: