FABian® - Cell selection device

Cell Selection Process using FABian®

First, FABian® is equipped with the Fab-TACS® Auto column filled with a Strep-Tactin® coated cell-grade agarose matrix (see fig. below) and reagent tubes containing the sample and the required selection reagents. FABian® starts the cell selection process by loading low-affinity Strep-tagged Fab fragments onto the matrix, e.g. a CD3 Fab-Strep for the selection of T cells. Subsequently, FABian® passes whole blood, mouse spleen cells or other single-cell suspensions through the column. Target cells adhere to the affinity matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are washed away efficiently.

Finally, FABian® loads biotin onto the column which causes the elution of the target cells and the Fab-Streps spontaneously self-dissociate from the cell surfaces due to their reduced affinity. Thus, the innovative Fab-TACS® procedure delivers label-free, non-activated target cells in a standardized manner of highly reproducible quality from a variety of single cell suspensions, e.g. whole blood or buffy coat allowing a multitude of downstream applications.
Key features

Easy handling:

  1. Switch on the device for initialization process
  2. Insert the column, filled with the matrix (here CD81 Fab-Strep for PBMC-like isolation)
  3. Place the tube rack with the tubes on the round about
  4. Choose program
  5. Press "go" and the selection process runs automatically
  6. Once the program is completed, take your selected target cells from the last tubes

Advantages at a glance:

  • Mild Fab-based immune affinity chromatography
  • Very high yields and purity (> 90%)
  • Minimally manipulated target cells, minimal cell stress
  • Highly reproducible
  • Automatic process
  • Compact & light
  • Embedded system, no external computer needed
  • Magnetic-free process
  • Various markers (Fab-fragments) available
  • Development of Fab fragments for customized selections possible
Comparison of PBMC isolation: density gradient vs FABian®

 

FABian® - Application data

Isolation of PBMCs and T cells from human whole blood
Using the FABian® platform, IBA Lifesciences has developed a unique approach for preparing Ficoll gradient-equivalent PBMC fractions in an automated and standardized manner. This process delivers PBMCs of highly reproducible quality from healthy as well as patient donors without the need for Ficoll density centrifugation – a major advance in cell selection for clinical and routine testing as well as research applications.
IBA Lifesciences offers FABian®-based automatic cell selection for specialized cell populations incl. T cells and other cell types. We are continuously expanding our cell selection applications as well as providing fully customized services for our customers.

For more information please visit www.fabian-online.com or watch our video.

Please download your application note of interest:

FABian® – Lymphocyte isolation

FABian® – Lymphocyte isolation Supplementary

FABian® vs Density gradient - Workflow

PBMC like cell selection by a Fab-based immune affinity chromatography

The first row of the image shows a 0.12 % share of PBMCs (CD81-positive cells) in the whole blood sample before the selection process.
The second row presents the results of the positive fraction after selection, indicating an excellent purity of 95 % and a high yield of the PBMCs (targeted CD81 positive cells).

FABian® in use

 

See how FABian® is used in the department of Experimental Transfusion Medicine at TU Dresden:

Watch now!