FABian® - Cell selection device

Cell Selection Process using FABian®

First, FABian® is equipped with a pre-made column filled with a Strep-Tactin® coated cell-grade agarose matrix (see fig. below) and max. 11 tubes containing the required selection reagents. FABian® starts the automated cell selection process by adding a specific Strep-tagged Fab fragment to the matrix. Subsequently, FABian® passes whole blood or other single-cell suspensions through the column. Target cells bind to the matrix based on the binding specificity of the loaded Fab fragment and non-target cells are washed away.
In the final step, FABian® adds D-biotin to the column, which causes elution of the Strep-tagged Fab from the matrix as well as dissociation of the Fab fragments from the cell surface. The label-free authentic target cells are now selected in high yields and purity. Remarkably, FABian® enables the selection of even small cell populations with high purity directly from whole blood.
Key features

Easy handling:

  1. Switch on the device for initialization process
  2. Insert the column, filled with the matrix (here CD81 Fab-Strep for PBMC-like isolation)
  3. Place the tube rack with the tubes on the round about
  4. Choose program
  5. Press "go" and the selection process runs automatically
  6. Once the program is completed, take your selected target cells from the last tubes

Advantages at a glance:

  • Mild Fab-based immune affinity chromatography
  • Very high yields and purity (> 90%)
  • Minimally manipulated target cells, minimal cell stress
  • Highly reproducible
  • Automatic process
  • Compact & light
  • Embedded system, no external computer needed
  • Magnetic-free process
  • Various markers (Fab-fragments) available
  • Development of Fab fragments for customized selections possible
Comparison of PBMC isolation: density gradient vs FABian®

 

FABian® - Application data

Isolation of PBMCs and T cells from human whole blood
Using the FABian® platform, IBA Lifesciences has developed a unique approach for preparing Ficoll gradient-equivalent PBMC fractions in an automated and standardized manner. This process delivers PBMCs of highly reproducible quality from healthy as well as patient donors without the need for Ficoll density centrifugation – a major advance in cell selection for clinical and routine testing as well as research applications.
IBA Lifesciences offers FABian®-based automatic cell selection for specialized cell populations incl. T cells and other cell types. We are continuously expanding our cell selection applications as well as providing fully customized services for our customers.

For more information please visit www.fabian-online.com or watch our video.

Please download your application note of interest:

FABian® – Lymphocyte isolation

FABian® – Lymphocyte isolation Supplementary

FABian® vs Density gradient - Workflow

PBMC like cell selection by a Fab-based immune affinity chromatography

The first row of the image shows a 0.12 % share of PBMCs (CD81-positive cells) in the whole blood sample before the selection process.
The second row presents the results of  the positive fraction after selection, indicating an excellent purity of 95 % and a high yield of the  PBMCs (targeted CD81 positive cells).

FABian® in use

 

See how FABian® is used in the department of Experimental Transfusion Medicine at TU Dresden:

Watch now!