Prevents biotinylated proteins from unspecific binding to Strep-Tactin® columns during Strep-tag® protein purification.
D-Biotin (not suitable for standard Strep-tag® applications)
Streptavidin, lyophilized (not suitable for Strep-tag® applications):
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket, as compared to native streptavidin.