6-8000-116
CD3/CD62L/CD45RA Fab-TACS® Magnetic Microbead Starter Kit, human
Label-free isolation of naive or central memory T cells from PBMCs
Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD3/CD62L/CD45RA Fab-TACS® Magnetic Microbead Starter Kit allows label-free isolation of CD3+ central memory (CD3+CD62L+CD45RA-) or naive (CD3+CD62L+CD45RA+) T cells from peripheral blood mononuclear cells (PBMCs).
Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) are incubated with Strep-Tactin® Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away. In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity. After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications.
Advantages:
• High purity of > 90%
• Scalable reagents
• Label-free cells due to low affinity Fab fragments
• Non-activated cells
• High purity of > 90%
• Scalable reagents
• Label-free cells due to low affinity Fab fragments
• Non-activated cells
The kit contains:
• Strep-Tactin® Magnetic Microbeads
• CD3 Fab-Strep, human
• CD62L Fab-Strep, human
• CD45RA Fab-Strep, human
• 10x Buffer CI
• Biotin stock solution
• Strep-Tactin® Magnetic Microbeads
• CD3 Fab-Strep, human
• CD62L Fab-Strep, human
• CD45RA Fab-Strep, human
• 10x Buffer CI
• Biotin stock solution
| Capacity: | 1.5 x 10^8 total cells |
|---|
| Storage: | 2-8 °C |
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| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
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