6-3301-002

CD4 Fab-TACS® Agarose Column Starter Kit, mouse

Label-free isolation of T helper cells from murine splenocytes

€144.00*

Content
Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD4 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of murine CD4+ T helper cells from splenocytes or other cell suspensions. CD4 is a co-receptor that associates with the T cell receptor and interacts with MHC class II molecules.
Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:
• Highly pure cells
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity Fab fragments

Specifications

Capacity: 1 x 10^8 total/2x10^7 target cells per column

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

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