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CD4 Fab-TACS® Magnetic Microbead Starter Kit, human

Label-free isolation of T helper cells from PBMCs

€83.00*

Content
Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD4 Fab-TACS® Magnetic Microbead Starter Kit allows label-free isolation of human CD4+ T helper cells from peripheral blood mononuclear cells (PBMCs). CD4 is a co-receptor that associates with the T cell receptor and interacts with MHC class II molecules.
Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) are incubated with Strep-Tactin® Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away. In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity. After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications.

Advantages:
• High purity of > 90%
• Scalable reagents
• Label-free cells due to low affinity Fab fragments
• Non-activated cells


For total cell numbers higher than 1 x 108, we recommend our Strep-Tactin® TACS Agarose columns that have a capacity of 1 x 109 total cells and can bind up to 1 x 108 target cells.

Specifications

Capacity: 5 x 10^7 total cells

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Blue ice

Accessories

StrepMan Magnet
Permanent magnet for cell isolations using Strep-Tactin® Magnetic Microbeads

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CD4 Fab-TACS® Agarose Column Starter Kit, human
Label-free isolation of T helper cells from whole blood, buffy coat or PBMCs