Applications for T cell stimulation and expansion with Streptamers®

T cell expansion after stimulation with CD3/CD28 Streptamers®

Human T cells isolated with the CD3 Fab Streptamer® Isolation Kit MB (6-8000-201) were cultured for 13 days with the CD3/CD28 Streptamer® premix for cell expansion. Fold expansion was measured.

Microscopy of T cells in culture showing cluster formation on day 3 after stimulation with CD3/CD28 Streptamers® for cell expansion

Human T cells isolated with the CD3 Fab Streptamer® Isolation Kit MB (6-8000-201) were cultured with (A) or without (B) CD3/CD28 Streptamers® for cell expansion.

T cell activation markers CD25 and CD69 after stimulation with CD3/CD28 Streptamers® for cell expansion

Human T cells isolated with CD3 Fab Streptamer® Isolation Kit MB (6-8000-201) were cultured for 6 days. Activation markers CD25 or CD69 were accessed in pregated CD3+ T cells using Flow Cytometry. Dead cells were excluded from the analysis using DAPI.

T cell proliferation after stimulation with CD3/CD28 Streptamers® for cell expansion

Human T cells were isolated with CD3 Fab Streptamer® Isolation Kit MB (6-8000-201), stained with CFSE abd cultured in a 48 well plate for 5 days. Proliferation was measured in pregated CD4+ (upper panel) or CD8+ T cells (lower panel) using Flow Cytometry. Dead cells were excluded from the analysis using Pi.