Alte Zweige > Protein Production & Assays > Expression vectors for classic cloning for E.coli, mammalian and yeast expression

Classic Cloning Vectors

Cloning vectors for expression in E. coli and mammalian cells that provide different protease cleavage sites or chloramphenicol resistance.

IBA Expression Vectors:

E. coli

For recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA) are available. The choice of the best expression vector depends on the characteristics of the protein. Due to the identical multiple cloning sites different vector backbones can be easily tested. pASK-IBA vectors are available with different protease cleavage sites (Tev, Factor Xa, Thrombin and Enterokinase) or with chloramphenicol resistance gene.

Mammalia

Expression in mammalian cells can be achieved with the CMV promoter based expression vectors (pEXPR-IBA) for high protein yields in a wide range of mammalian cells. These vectors provide a N-terminal Strep-tag®II followed by a special protease cleavage site for removal of the tag after purification.

For an overview on the classic cloning vector portfolio, click the Additional Expression Vector Table.

 

More vector variants

The StarGate expression system provides even more expression vectors (Acceptor Vectors) for E. coli (pASG-IBA; pPSG-IBA), mammalia (pDSG-IBA, pESG-IBA, pCSG-IBA), yeast (pYSG-IBA) and insect cells (pLSG-IBS). All providing different characteristics for expression (different promoters, secretion signal) or purification (different affinity tags).

In addition, with its new mammalian expression system MEXi IBA offers a complete expression system including vectors, HEK293E cell line and medium.

Since the StarGate vectors are implemented in a universal cloning strategy for all vectors the switch between different vector backbones and even hosts is easy and straightforward. For more information see StarGate cloning.

For an overview on the StarGate vector portfolio, click the Expression Vector Table.

 

!In cases where the optimal expression system (affinity tag) is not known and different affinity tags have to be tested, our “Two-step-cloning” via pENTRY-IBA variant within the StarGate cloning system might be advantageous.