Desthiobiotin for elution of Strep-tag® II proteins from Strep-Tactin®. It displaces Strep-tag® II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution.
Desthiobiotin powder for preparation of elution buffer or ready-to-use buffer (see 2-1000-025). Completely dissolve 0.107 g Desthiobiotin in 200 ml of a buffer compatible with the recombinant protein (e. g. buffer W).
Molecular weight: 214,3 g/mol.
For more information click the data sheet under downloads.
Do not use for elution from Strep-Tactin®XT resin!