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Contents: 2 g Cat.no: 2-1016-002 Price: USD 78.00  

Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H)

It binds very tightly (irreversibly) to Streptavidin and Strep-Tactin® and with very high affinity (but reversible) to Strep-Tactin®XT. Therefore biotin allows the efficient elution of Strep-tagged proteins from Strep-Tactin®XT, MagStrep "type3" XT beads. Biotin binds irreversible to Strep-Tactin®, therefore the resin cannot be regenerated. Strep-Tactin®XT can be regenerated after elution with biotin by addition of Buffer XT-R.

How to prepare a 50 mM biotin solution for elution? https://www.iba-lifesciences.com/tips-and-tricks.html