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pASK-IBA6C vector

Contents: 5 µg Cat.no: 2-1325-000 Price: USD 225.00  

The pASK-IBA6C vector allows the expression of Strep-tag®-fusion-proteins in E.coli.

The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the Strep-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any E. coli strain because the tet-promoter works independently from the genetic background of E.coli. The additional factor Xa cleavage site allows the removal of the Strep-tag®.

  • promoter from bp 37 to 72
  • forward primer binding site from bp 57 to 76
  • OmpA signal sequence from bp 139 to 201
  • Strep-tag® from bp 202 to 231
  • factor Xa cleavage site from bp 232 to 243
  • multiple cloning site from bp 244 to 320
  • reverse primer binding site from bp 388 to 404
  • f1 origin from bp 417 to 855
  • CamR resistance gene from bp 977 to 1636
  • tet-repressor from bp 1649 to 2272