Buffer R is a Strep-Tactin® Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin®. HABA (2-[4 -hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin®. The yellow HABA solution turns red upon binding to Strep-Tactin® clearly indicating the regeneration process and activity status of the column.
Visualization of column regeneration and activity status by color change.
Do not use for regeneration of Strep-Tactin®XT resin!