StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coli.
- Optimized for MEXi (Mammalian expression system IBA)
The expressed recombinant protein will be secreted in the cell culture medium (BM40 signal sequence).
This vector contains a N-terminal Twin-Strep-tag.