The kit allows the selection of CD4+ T cells directly from whole blood, buffy coat or PBMCs.
The Kit includes:
- 10x Fab-TACS® Gravity column (Capacity: 1x 10^8 target cells per column)
- CD4 Fab-Strep, human
- Buffer CI (10x)
- Biotin stock solution
- 2x Fab-TACS® Gravity Adapter
*Cat. No. 6-3202-010 replaces Cat. No. 6-6202-010 (the products are identical)
The Fab-TACS® Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, whole blood or other blood preparations pass through the column. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are now ready for further downstream applications.