The kit allows the selection of exosomes from cell culture supernatant, serum and plasma.
The Kit includes:
- 10x Fab-TACS® Gravity column (Capacity: 5x10^10 targeted exosomes per column; 10ml cell culture supernataqnt per column)
- CD81 Fab-Strep, human
- Biotin stock solution
- 2x Fab-TACS® Gravity Adapter
The Fab-TACS® Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged CD81 Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, the sample passes through the column. Targeted exosomes adhere to the matrix based on the exclusive binding of the CD81 Fab-Strep to the targeted exosome. Non-targeted particles are efficiently washed away. In a final step, the addition of biotin causes the elution of the targeted exosomes and the CD81 Fab-Streps. After elution, the CD81 Fab-Streps self-dissociate from the exosome surfaces. The label-free authentic targeted exosomes are now ready for further downstream applications.