The kit allows the selection of exosomes from cell culture supernatant, serum and plasma.
The Kit includes:
- 4x Fab-TACS® Gravity column (Capacity: 5x10^10 targeted exosomes per column; 10ml cell culture supernatant per column)
- CD9 Fab-Strep, human
- CD81 Fab-Strep, human
- Biotin stock solution
- 1x Fab-TACS® Gravity Adapter
- 4x Minisart® Syringe Filter, Polyethersulfone, 0.22 µm*
- 4x Minisart® Syringe Filter, Cellulose Acetate, 0.2 µm*
The Fab-TACS® Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged CD9 or CD81 Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, the sample passes through the column. Targeted exosomes adhere to the matrix based on the exclusive binding of the CD9 or CD81 Fab-Strep to the targeted exosome. Non-targeted particles are efficiently washed away. In a final step, the addition of biotin causes the elution of the targeted exosomes and the CD9 or CD81 Fab-Streps. After elution, the CD9 or CD81 Fab-Streps self-dissociate from the exosome surfaces. The label-free authentic targeted exosomes are now ready for further downstream applications.
*The filters are included as long as supply lasts.