The kit allows the selection of CD4+ T cells from mouse splenocytes.
The Kit includes:
- 4x Fab-TACS® Gravity column (Capacity: 1x10^8 target cells each)
- 4x CD4 Fab-Strep, mouse
- Buffer CI (10x)
- Biotin stock solution
- 1x Fab-TACS® Gravity Adapter
*Cat. No. 6-3401-004 replaces Cat. No. 6-6401-004 (the products are identical)
The Fab-TACS® Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, single-cell tissue suspensions pass through the column. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are now ready for further downstream applications. Find more information here https://www.iba-lifesciences.com/fab-tacs-gravity-technology.html.