Home > CD45RA Fab Streptamer® Isolation Kit MB, human

CD45RA Fab Streptamer® Isolation Kit MB, human

Contents: for isolation out of 1x10^9 cells with magnetic microbeads (MB); to be used with a permanent magnet Cat.no: 6-8000-208 Price: USD 663.00  

For isolation of CD45RA+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer® components for positive cell isolation with magnetic microbeads* and for removal of Streptamers from isolated cells.

1.) Strep-Tactin® Magnetic Microbeads for Fab Streptamers; 2 vials for 5x10^8 cells each

2.) CD45RA Fab-Strep, human for isolation of T-cells; 2 vials for 5x10^8 cells each

3. ) D-Biotin stock solution for Streptamer® technology; 2x 1 ml

4.) Buffer IS, Streptamer® Washing Buffer, 10x concentrated; 50 ml

This product requires dry ice shipment and is shipped directly from our Headquarters in Germany.

*Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 ml and 2 x 50 ml Falcon tubes.

Principle of Selection:

Isolation of human CD45RA+ T cells is performed by positive selection. PBMCs are labeled with magnetic CD45RA Fab Streptamers®. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD45RA positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position). The magnetic Streptamers migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing.

The resulting cell preparation is highly enriched with CD45RA+ T cells with a purity of more than 90%. No depletion steps and no columns are needed.