Transfection with common lipidic or polycationic reagents can be enhanced by magnetic assistance ("Magnet Assisted Lipofection"). In this case, the nucleic acid has to be combined with MA Lipofection Enhancer in the presence of a common lipid based or polycationic reagent. The formulation of MA Lipofection Enhancer, which contains magnetic nanoparticles (MagTag®), can be used with IBA?s lipofection reagent IBAfect or any other commercially available lipofection reagent.
The MA Lipofection Enhancer is suitable for adherent cells; for suspension cells MATra-S Immobilizer is required in addition. Moreover, the MA Lipofection Enhancer can be used for viral transfection. For the transfection procedure a MATra magnet plate is essential.
All MATra reagents with magnetic beads have to be stored distant (> 10 cm) from strong magnetic fields like the Magnet Plates at all times!!! Otherwise agglutination of the beads may lead to complete loss of transfection function.