Fab-TACS Gravity - Applications
The Fab-TACS technology provides a vital benefits for your purified cells and following applications. In this section, we demonstrate the TACS benefits with convincing data:
Isolation of CD3+ and CD4+ cells directly from whole blood. Cells were stained either with CD3-APC (OKT-3) or CD4-PE (OKT-4) and analyzed by flow cytometry. Dead cells were excluded from analysis using DAPI staining. Doublet and debris discrimination were performed using different FSC/SSC signals. Fab-TACS technology ensures highest purity independent of the starting material.
APC-Fluorescence after Fab-TACS Gravity CD3 isolation with CD3-FITC and Strep-Tactin®-APC. The target cells do not bind the staining reagent since all isolation reagents dissociated from the cell. Intentionally labeled cells bind the dye resulting in a fluorescence shift. Fab-TACS ensures authentic target cells.