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Related References

Applications of the Fab-TACS® technology

  1. Loading of Primary Human T Lymphocytes with Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Does Not Impair Their Activation after Polyclonal Stimulation.
    Marina Mühlberger, Harald Unterweger, Julia Band, Christian Lehmann, Lukas Heger, Diana Dudziak, Christoph Alexiou , Georey Lee  and Christina Janko. Cells2020, 9(2), 342; https://doi.org/10.3390/cells9020342

  2. An Evaluation of T‐Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation.
    Immanuel Andrä, Hanna Ulrich, Susi Dürr, Dominik Soll, Lynette Henkel, Corinne Angerpointner, Julia Ritter, Sabine Przibilla, Herbert Stadler, Manuel Effenberger, Dirk H. Busch, Matthias Schiemann. Cytometry Part A 97(3), January 2020. https://doi.org/10.1002/cyto.a.23964

Alternative applications for Fab-TACS® columns

In the following two papers Mühlberger et al. used Fab-TACS® columns to separate cells loaded with superparamagnetic iron oxide nanoparticles (SPIONs) from unbound SPIONs. The authors replaced the Fab-Streps supplied with our kits with a desthiobiotinylated anti-mouse CD90.2 antibody, which can also be eluted with biotin. This approach allowed them to successfully separate magnetic cells from magnetic particles with an antibody of their choice:

  1. Functionalization Of T Lymphocytes With Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles For Magnetically Controlled Immune Therapy.
    Mühlberger M, Janko C, Unterweger H, Friedrich RP, Friedrich B, Band J, Cebulla N, Alexiou C, Dudziak D, Lee G, Tietze R. International Journal of Nanomedicine Volume 14:8421-8432, October 2019. https://doi.org/10.2147/IJN.S218488

  2. Non-magnetic chromatographic separation of colloidally metastable superparamagnetic iron oxide nanoparticles and suspension cells.
    Mühlberger M, Janko C, Unterweger H, Band J, Schreiber E, Lehmann C, Dudziak D, Lee G, Alexiou C, Tietze R. Journal of Chromatography B, Volumes 1122-1123, August 2019. https://doi.org/10.1016/j.jchromb.2019.05.033

The development of the Fab-TACS® technology

  1. Efficient immunoaffinitychromatography of lymphocytesdirectly from whole blood.
    Fabian Mohr, Sabine Przibilla, Franziska Leonhardt, Christian Stemberger, Stefan Dreher,Thomas R. Müller, Simon P. Fräßle, Georg P. Schmidt, Marie-Luise Kiene, Herbert Stadler & Dirk H. Busch (2018). Scientific Reportsvolume 8, Article number: 16731 (2018). https://rdcu.be/b3gzn

Reviews

  1. Addressing the Manufacturing Challenges of Cell-Based Therapies.
    Miguel de Almeida Fuzeta, André Dargen de Matos Branco, Ana Fernandes-Platzgummer, Cláudia Lobato da Silva and Joaquim M. S. Cabral.Advances in Biochemical Engineering/Biotechnology 171, December 2019. https://link.springer.com/chapter/10.1007%2F10_2019_118