Fab-TACS Gravity - Manual Chromatography Columns for Cell Selection

Fab-TACS - Traceless Affinity Cell Selection from e.g. whole blood

Click to enlarge the figure

IBA’s unique Strep-tag®/Strep-Tactin® system is the platform for the novel Fab-based traceless affinity cell selection (Fab-TACS) of cells. Fab-TACS uses immune affinity chromatography based on CD-specific Fab-fragments for reversible capture and release of target cells. The innovative Fab-TACS procedure delivers label-free, non-activated target cells in a standardized manner of highly reproducible quality directly from whole blood or other blood preparations.

The Fab-TACS positive selection completely resigns the use of high affinity antibodies whose usage causes unfavorable effects like strong and almost irreversible binding to cells, cell stimulation as well as receptor blockade.

Watch this video to get further insights into the Fab-TACS technology: Click here!

Fab-TACS Gravity - Manual Chromatography Columns for Cell Selection

Get highly pure cell populations within a few
convenient steps (click to enlarge the figure)

The Fab-TACS Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, whole blood or other blood preparations pass through the column. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are now ready for further downstream applications.